Rabbit anti-Human TRAIP Polyclonal Antibody | anti-TRAIP antibody

TRAIP Antibody

Cat. Num. AAA9630606

• Gene Names: TRAIP; TRIP; RNF206
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TRAIP; Polyclonal Antibody; TRAIP Antibody; RING finger protein 206; RNF206; TRAF Interacting Protein; TRAF-interacting protein; Traip; TRAIP_HUMAN; TRIP; anti-TRAIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: TRAIP Antibody detects endogenous levels of TRAIP.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TRAIP antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human TRAIP.
• Conjugation: Unconjugated
• Post Translational Modifications: Autoubiquitinated.
• Subunit Structure: Binds TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6 is part of the receptor-TRAF signaling complex (By similarity). The C-terminus interacts with CYLD, however the interaction was not detected with the full-length protein. Interacts with POLK and POLN.
• Function: E3 ubiquitin ligase acting as a negative regulator of innate immune signaling. Inhibits activation of NF-kappa-B mediated by TNF. Negatively regulates TLR3/4- and RIG-I-mediated IRF3 activation and subsequent IFNB1 production and cellular antiviral response by promoting 'Lys-48'-linked polyubiquitination of TNK1 leading to its proteasomal degradation (By similarity). Involved in response to genotoxic lesions during genome replication. Promotes H2AX and RPA2 phosphorylation after replication-associated DNA damage and assists fork progression at UV-induced replication-blocking lesions during S phase. Has also been proposed to play a role in promoting translesion synthesis by mediating the assembly of 'Lys-63'-linked poly-ubiquitin chains on the Y-family polymerase POLN in order to facilitate bypass of DNA lesions and preserve genomic integrity. The function in translesion synthesis is controversial.
• Note: In the nucleus, found in close proximity to PCNA, suggesting localization at replication foci. After UV irradiation, rapidly localizes to sites of DNA damage, where it colocalizes with phosphorylated histone H2AX (gamma-H2AX) and RPA2.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-TRAIP antibody

Cytoplasm; Perinuclear region; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 40807469
• NCBI GeneID: 10293
• NCBI Accession #: NP_005870.2
• NCBI GenBank Nucleotide #: NM_005879.2
• UniProt Accession #: Q9BWF2; O00467; B5BU84; B5BUL3
• Molecular Weight: 53,294 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRAIP
• NCBI Official Synonym Full Names: TRAF interacting protein
• NCBI Official Symbol: TRAIP
• NCBI Official Synonym Symbols: TRIP; RNF206
• NCBI Protein Information: E3 ubiquitin-protein ligase TRAIP; ring finger protein 206; TRAF-interacting protein
• UniProt Protein Name: E3 ubiquitin-protein ligase TRAIP
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRAIP
• UniProt Synonym Gene Names: RNF206; TRIP
• UniProt Entry Name: TRAIP_HUMAN

NCBI Description

This gene encodes a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAIP: Inhibits activation of NF-kappa-B mediated by TNF.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Nucleolus; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: perinuclear region of cytoplasm; nucleolus

Molecular Function: protein binding; zinc ion binding; receptor signaling protein activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: cell proliferation; apoptosis; protein ubiquitination; signal transduction