Rabbit anti-Human FBXO5 Polyclonal Antibody | anti-FBXO5 antibody

FBXO5 Antibody

Cat. Num. AAA9630523

• Gene Names: FBXO5; EMI1; FBX5; Fbxo31
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: FBXO5; Polyclonal Antibody; FBXO5 Antibody; Early mitotic inhibitor 1; Emi 1; EMI1; F box only protein 5; F box protein 5; F box protein Fbx 5; F box protein Fbx5; F-box only protein 5; FBX5; FBX5_HUMAN; Fbxo31; fbxo5; anti-FBXO5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: FBXO5 Antibody detects endogenous levels of FBXO5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-FBXO5 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human FBXO5.
• Conjugation: Unconjugated
• Post Translational Modifications: Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression. Phosphorylated by RPS6KA2; increases and stabilizes interaction with CDC20 (By similarity).Ubiquitinated by the SCF(BTRC) complex following phosphorylation by PLK1. Undergoes both 'Lys-11' and 'Lys-48'-linked polyubiquitination by APC-FZR1 complex leading to degradation by proteasome during G1 phase. Degraded through the SCF(BTRC) complex; degradation occurs during oocyte maturation, between germinal vesicle breakdown (GVBD) and meiosis I, and is required for the meiosis I-meiosis II transition (By similarity).
• Subunit Structure: Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). Interacts with BTRC; mediates proteolysis by the SCF ubiquitin ligase complex leading to activation of APC in late mitosis and subsequent mitotic progression. Interacts with FZR1/CDH1 and the N-terminal substrate-binding domain of CDC20; prevents APC activation. Also interacts with EVI5 which blocks its phosphorylation by PLK1 and prevents its subsequent binding to BTRC and degradation. Interacts simultaneously with anaphase promoting complex (APC), through at least ANAPC2, CDC23, CDC27, the APC substrate GMNN and the APC activator FZR1. Interacts with UBE2S; interferes with the activity of UBE2S mainly by disrupting the dynamic electrostatic association between the C-terminal tail of UBE2S and ANAPC2. Interacts with RPS6KA2; cooperates to induce the metaphase arrest of early blastomeres; increases and stabilizes interaction of FBXO5 with CDC20 (By similarity).
• Function: Regulator of APC activity during mitotic and meiotic cell cycle. During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex. During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase. Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment. As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities. Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence. During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (By similarity). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (By similarity). Promotes migration and osteogenic differentiation of mesenchymal stem cells.
• Note: In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration (PubMed:11988738). In mitotic cells, localizes throughout the cell, particularly at the spindle (PubMed:15469984).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-FBXO5 antibody

Nucleus; Cytoplasm; Cytoskeleton; Spindle; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 215982802
• NCBI GeneID: 26271
• NCBI Accession #: NP_001135994.1
• NCBI GenBank Nucleotide #: NM_001142522.1
• UniProt Accession #: Q9UKT4; Q5TF47; Q8WV29; Q9UGC8; B3KNX5
• Molecular Weight: 50,146 Da
• NCBI Official Full Name: F-box only protein 5 isoform b
• NCBI Official Synonym Full Names: F-box protein 5
• NCBI Official Symbol: FBXO5
• NCBI Official Synonym Symbols: EMI1; FBX5; Fbxo31
• NCBI Protein Information: F-box only protein 5; F-box protein Fbx5; early mitotic inhibitor 1
• UniProt Protein Name: F-box only protein 5
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO5
• UniProt Synonym Gene Names: EMI1; FBX5
• UniProt Entry Name: FBX5_HUMAN