Rabbit anti-Human ATP5J Polyclonal Antibody | anti-ATP5J antibody

ATP5J Antibody

Cat. Num. AAA9629904

• Gene Names: ATP5J; F6; CF6; ATP5; ATPM; ATP5A
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ATP5J; Polyclonal Antibody; ATP5J Antibody; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit F6; ATP synthase-coupling factor 6; mitochondrial; ATP5; ATP5A; ATP5J_HUMAN; ATPase subunit F6; ATPM; CF6; F6; anti-ATP5J antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ATP5J Antibody detects endogenous levels of ATP5J.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ATP5J antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ATP5J.
• Conjugation: Unconjugated
• Similarity: Belongs to the eukaryotic ATPase subunit F6 family.
• Subunit Structure: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and MP68 (By similarity).
• Function: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ATP5J antibody

Mitochondrion; Mitochondrion inner membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 114688
• NCBI GeneID: 522
• UniProt Accession #: P18859; J3KQ83
• Molecular Weight: 108
• NCBI Official Full Name: ATP synthase-coupling factor 6, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
• NCBI Official Symbol: ATP5J
• NCBI Official Synonym Symbols: F6; CF6; ATP5; ATPM; ATP5A
• NCBI Protein Information: ATP synthase-coupling factor 6, mitochondrial; ATPase subunit F6; proliferation-inducing protein 36; mitochondrial ATP synthase, subunit F6; mitochondrial ATPase coupling factor 6; mitochondrial ATP synthase, coupling factor 6; ATP synthase, H+ transporti
• UniProt Protein Name: ATP synthase-coupling factor 6, mitochondrial
• Protein Family: ATP synthase-coupling factor
• UniProt Gene Name: ATP5J
• UniProt Synonym Gene Names: ATP5A; ATPM; ATPase subunit F6
• UniProt Entry Name: ATP5J_HUMAN

NCBI Description

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.[provided by RefSeq, Jun 2010]

Uniprot Description

ATP5J: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes. Belongs to the eukaryotic ATPase subunit F6 family.

Protein type: EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Mitochondrial

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: transporter activity; ATPase activity; transmembrane transporter activity; hydrogen ion transmembrane transporter activity

Biological Process: cellular metabolic process; substantia nigra development; mitochondrial ATP synthesis coupled proton transport