Rabbit anti-Human, Mouse NANOS1 Polyclonal Antibody | anti-NANOS1 antibody

NANOS1 Antibody

Cat. Num. AAA9629902

• Gene Names: NANOS1; NOS1; SPGF12
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NANOS1; Polyclonal Antibody; NANOS1 Antibody; EC Rep1a; EC_Rep1a; NANO1_HUMAN; NANOS 1; Nanos homolog 1 (Drosophila); Nanos homolog 1; Nanos Homologue 1 (NANOS1); Nos 1; NOS-1; Nos1; OTTHUMP00000046853; Rep 1a; Rep1a; anti-NANOS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: NANOS1 Antibody detects endogenous levels of NANOS1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NANOS1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human NANOS1.
• Conjugation: Unconjugated
• Similarity: The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein. The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN. Belongs to the nanos family.
• Subunit Structure: Interacts with PUM2, SNAPIN and CTNNB1. Interacts (via N-terminal region) with CTNND1. Interacts with DDX20 (via N-terminal region).
• Tissue Specificity: Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
• Function: May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
• Note: Colocalizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-NANOS1 antibody

Cytoplasm; Perinuclear region; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 41688589
• NCBI GeneID: 340719
• UniProt Accession #: Q8WY41
• Molecular Weight: 30,230 Da
• NCBI Official Full Name: Nanos homolog 1
• NCBI Official Synonym Full Names: nanos homolog 1 (Drosophila)
• NCBI Official Symbol: NANOS1
• NCBI Official Synonym Symbols: NOS1; SPGF12
• NCBI Protein Information: nanos homolog 1; NOS-1; EC_Rep1a
• UniProt Protein Name: Nanos homolog 1
• Protein Family: Nanos
• UniProt Gene Name: NANOS1
• UniProt Synonym Gene Names: NOS1; NOS-1
• UniProt Entry Name: NANO1_HUMAN

NCBI Description

This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015]

Uniprot Description

NANOS1: May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion. Belongs to the nanos family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein binding; translation repressor activity; zinc ion binding; RNA binding

Biological Process: cell migration; negative regulation of translation

Disease: Spermatogenic Failure 12