Rabbit anti-Human, Mouse NCAPD2 Polyclonal Antibody | anti-NCAPD2 antibody

NCAPD2 Antibody

Cat. Num. AAA9629823

• Gene Names: NCAPD2; CNAP1; CAP-D2; hCAP-D2
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NCAPD2; Polyclonal Antibody; NCAPD2 Antibody; 2810406C15Rik; 2810465G24Rik; CAP-D2; CAPD2; CEP2; CEP250; Chromosome condensation-related SMC-associated protein 1; Chromosome-associated protein D2; CNAP1; CND1; Condensin; Condensin complex subunit 1; eg7; hCAP-D2; hCAPD2; KIAA0159; mKIAA0159; non-SMC condensin I complex; subunit D2; XCAP D2 homolog; anti-NCAPD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: NCAPD2 Antibody detects endogenous levels of NCAPD2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NCAPD2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human NCAPD2.
• Conjugation: Unconjugated
• Similarity: The C-terminal domain interacts with histones H1 and H3, and may be responsible for condensin complex targeting to mitotic chromosomes. This domain is independent from the bipartite nuclear localization signal, although they are contained within the same region. Belongs to the CND1 (condensin subunit 1) family.
• Post Translational Modifications: Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPH and NCAPG subunits, activates the condensin complex and is required for chromosome condensation (By similarity).
• Subunit Structure: Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: NCAPH/BRRN1, NCAPD2/CAPD2 and NCAPG. Interacts with histones H1 and H3.
• Function: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain. May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.
• Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-NCAPD2 antibody

Nucleus; Cytoplasm; Chromosome; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 259016362
• NCBI GeneID: 9918
• UniProt Accession #: Q15021; Q8N6U3; D3DUR4
• Molecular Weight: 1401
• NCBI Official Full Name: Condensin complex subunit 1
• NCBI Official Synonym Full Names: non-SMC condensin I complex, subunit D2
• NCBI Official Symbol: NCAPD2
• NCBI Official Synonym Symbols: CNAP1; CAP-D2; hCAP-D2
• NCBI Protein Information: condensin complex subunit 1; XCAP-D2 homolog; chromosome-associated protein D2; chromosome condensation related SMC associated protein 1; chromosome condensation-related SMC-associated protein 1
• UniProt Protein Name: Condensin complex subunit 1
• Protein Family: Condensin complex
• UniProt Gene Name: NCAPD2
• UniProt Synonym Gene Names: CAPD2; CNAP1; KIAA0159; hCAP-D2
• UniProt Entry Name: CND1_HUMAN

Uniprot Description

CNAP1: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain. Belongs to the CND1 (condensin subunit 1) family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: nucleoplasm; nuclear chromosome; membrane; pronucleus; cytoplasm; condensin complex; condensin core heterodimer; condensed chromosome; cytosol; nucleus

Molecular Function: protein binding; histone binding

Biological Process: cell division; mitotic chromosome condensation; mitotic cell cycle