Rabbit anti-Human, Mouse Pirh2 Polyclonal Antibody | anti-RCHY1 antibody

Pirh2 Antibody

Cat. Num. AAA9629596

• Gene Names: RCHY1; ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Pirh2; Polyclonal Antibody; Pirh2 Antibody; Androgen receptor N terminal interacting protein; Androgen receptor N-terminal-interacting protein; ARNIP; CH-rich-interacting match with PLAG1; CHIMP; E3 ubiquitin-protein ligase Pirh2; hARNIP; hPirh2; p53 induced protein with a RING H2 domain; p53-induced RING-H2 protein; PIRH2E; PIRH2F; PRO1996; RCHY1; Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase; RING finger and CHY zinc finger domain-containing protein 1; RING finger protein 199; RNF199; ZCHY; ZFP 363; zinc finger CHY type; Zinc finger protein 363; ZN363_HUMAN; ZNF363; anti-RCHY1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Pirh2 Antibody detects endogenous levels of total Pirh2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RCHY1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Post Translational Modifications: Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability.
• Subunit Structure: Monomer and homodimer. Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, CDKN1B, COPE, UBE2D2 and GORAB/NTKLBP1.
• Function: Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RCHY1 antibody

Nucleus; Nucleus speckle; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 32700008
• NCBI GeneID: 25898
• UniProt Accession #: Q96PM5; Q2KN33; Q59GN7; Q86X26; B3KRG3; C7E541; C7E542; C7E543; D3YRV2; E7EMC8; E7ETW5; J3KPI0
• Molecular Weight: 261
• NCBI Official Full Name: RING finger and CHY zinc finger domain-containing protein 1
• NCBI Official Synonym Full Names: ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: RCHY1
• NCBI Official Synonym Symbols: ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996
• NCBI Protein Information: RING finger and CHY zinc finger domain-containing protein 1; zinc finger, CHY-type; RING finger protein 199; zinc finger protein 363; E3 ubiquitin-protein ligase Pirh2; CH-rich interacting match with PLAG1; p53-induced protein with a RING-H2 domain; andro
• UniProt Protein Name: RING finger and CHY zinc finger domain-containing protein 1
• Protein Family: RING finger and CHY zinc finger domain-containing protein
• UniProt Gene Name: RCHY1
• UniProt Synonym Gene Names: ARNIP; CHIMP; PIRH2; RNF199; ZNF363; hPirh2
• UniProt Entry Name: ZN363_HUMAN

NCBI Description

The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

RCHY1: Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and p27Kip1. Preferentially acts on tetrameric p53/TP53. Contributes to the regulation of p27Kip1 and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity. Monomer and homodimer. Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, p27Kip1, COPE, UBE2D2 and GORAB/NTKLBP1. Up-regulated during the S phase of the cell cycle. Expressed at low levels during G phase. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Nuclear receptor co-regulator; EC 6.3.2.-; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; p53 binding; ubiquitin-protein ligase activity; ligase activity; receptor binding

Biological Process: protein autoubiquitination; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination