Rabbit RNF139 Polyclonal Antibody | anti-RNF139 antibody

RNF139 Antibody

Cat. Num. AAA9629381

• Gene Names: RNF139; RCA1; TRC8; HRCA1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RNF139; Polyclonal Antibody; RNF139 Antibody; E3 ubiquitin-protein ligase RNF139; HRCA1; RCA1; Ring finger protein 139; Translocation in renal carcinoma on chromosome 8 protein; TRC8; anti-RNF139 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RNF139 Antibody detects endogenous levels of total RNF139.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RNF139 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The RING-type zinc finger domain mediates ubiquitin ligase activity.
• Post Translational Modifications: Autoubiquitinated. Ubiquitination is induced by sterol and leads to ist degradation via the ubiquitin-proteasome pathway.
• Subunit Structure: Interacts with MHC class I and HM13. Interacts with VHL. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts with SREBF2 (via C-terminal domain). Interacts with SCAP; the interaction inhibits the interaction of SCAP with SEC24B and hampering the ER to Golgi transport of the SCAP/SREBP complex. Interacts with SEC24B. Interacts with INSIG1 and INSIG2. Interacts with EIF3F and EIF3H; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with XBP1 isoform 1; the interaction induces ubiquitination and degradation of XBP1 isoform 1.
• Tissue Specificity: Highly expressed in testis, placenta and adrenal gland. Moderate expression in heart, brain, liver, skeletal muscle and pancreas, and low expression in lung and kidney.
• Function: E3-ubiquitin ligase; acts as a negative regulator of the cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP/SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination. May function as a signaling receptor.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RNF139 antibody

Endoplasmic reticulum membrane; Multi-pass membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 21314654
• NCBI GeneID: 11236
• NCBI Accession #: NP_009149.2
• NCBI GenBank Nucleotide #: NM_007218.3
• UniProt Accession #: Q8WU17; O75485; Q7LDL3; B3KMD5
• Molecular Weight: 75,994 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF139
• NCBI Official Synonym Full Names: ring finger protein 139
• NCBI Official Symbol: RNF139
• NCBI Official Synonym Symbols: RCA1; TRC8; HRCA1
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF139; multiple membrane spanning receptor TRC8; patched related protein translocated in renal cancer; translocation in renal carcinoma on chromosome 8 protein
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF139
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF139
• UniProt Entry Name: RN139_HUMAN

NCBI Description

The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF139: E3-ubiquitin ligase; acts as a negative regulator of the cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP/SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination. May function as a signaling receptor. Defects in RNF139 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. A chromosomal aberration involving RNF139 has been found in a lymphoblastoid cell line established from a family with renal cell carcinoma and thyroid carcinoma. Translocation (3;8)(q14.2;q24.1) with FHIT. RNF139 is found to be fused to FHIT and disrupted within the sterol-sensing domain. In contrast, the FHIT coding region is maintained and expressed. Sporadic cases of renal carcinoma, where an acquired mutation in RNF139 results in the duplication of 12 nucleotides in the 5'-UTR, has also been identified.

Protein type: Ligase; Membrane protein, multi-pass; EC 6.3.2.-; EC 6.3.2.19; Membrane protein, integral; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; protease binding; small conjugating protein ligase activity; zinc ion binding; ubiquitin-protein ligase activity; receptor activity; ligase activity

Biological Process: negative regulation of cell proliferation; protein destabilization; regulation of protein ubiquitination; negative regulation of translation; protein ubiquitination

Disease: Renal Cell Carcinoma, Nonpapillary