Rabbit anti-Human, Mouse EXOSC5 Polyclonal Antibody | anti-EXOSC5 antibody

EXOSC5 Antibody

Cat. Num. AAA9629220

• Gene Names: EXOSC5; p12B; RRP46; RRP41B; Rrp46p; hRrp46p
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: EXOSC5; Polyclonal Antibody; EXOSC5 Antibody; Chronic myelogenous leukemia tumor antigen 28; CML28; EXOS5_HUMAN; EXOSC 5; Exosc5; Exosome complex component rrp46; exosome complex exonuclease RRP46; Exosome component 5; exosome component Rrp46; hRrp46p; p12B; Ribosomal RNA processing protein 46; S. cerevisiae; homolog of; Ribosomal RNA-processing protein 46; RRP41B; RRP46; Rrp46p; anti-EXOSC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: EXOSC5 Antibody detects endogenous levels of total EXOSC5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-EXOSC5 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the RNase PH family.
• Subunit Structure: Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core (Exo-9) complex which is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits specifically containing the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and peripheral S1 domain-containing components EXOSC1, EXOSC2 and EXOSC3 located on the top of the ring structure. Interacts with EXOSC1. Interacts with GTPBP1. Interacts with ZC3HAV1. Interacts with DDX17 only in the presence of ZC3HAV1 in an RNA-independent manner.
• Tissue Specificity: Highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis.
• Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-EXOSC5 antibody

Nucleus; Cytoplasm; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 14285757
• NCBI GeneID: 56915
• UniProt Accession #: Q9NQT4; Q32Q81; Q8NG16; Q96I89
• Molecular Weight: 235
• NCBI Official Full Name: Exosome complex component RRP46
• NCBI Official Synonym Full Names: exosome component 5
• NCBI Official Symbol: EXOSC5
• NCBI Official Synonym Symbols: p12B; RRP46; RRP41B; Rrp46p; hRrp46p
• NCBI Protein Information: exosome complex component RRP46; exosome component Rrp46; exosome complex exonuclease RRP46; ribosomal RNA-processing protein 46; chronic myelogenous leukemia tumor antigen 28
• UniProt Protein Name: Exosome complex component RRP46
• Protein Family: Exosome complex component
• UniProt Gene Name: EXOSC5
• UniProt Synonym Gene Names: CML28; RRP46
• UniProt Entry Name: EXOS5_HUMAN

Uniprot Description

EXOSC5: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. Belongs to the RNase PH family.

Protein type: Ribonuclease; Nucleolus; EC 3.1.13.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; nucleolus; exosome (RNase complex); nucleus; cytosol

Molecular Function: protein binding; 3'-5'-exoribonuclease activity; exoribonuclease activity; RNA binding

Biological Process: gene expression; DNA deamination; defense response to virus; rRNA processing; mRNA catabolic process, deadenylation-dependent decay