Rabbit anti-Human, Mouse TFB2M Polyclonal Antibody | anti-TFB2M antibody
TFB2M Antibody
Gene Names
TFB2M; Hkp1; mtTFB2
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TFB2M; Polyclonal Antibody; TFB2M Antibody; N''-adenosyl(rRNA) dimethyltransferase 2; S adenosylmethionine 6 N'; N' adenosyl(rRNA) dimethyltransferase 2; Dimethyladenosine transferase 2; dimethyladenosine transferase 2 mitochondrial; FLJ22661; FLJ23182; h mtTFB; h mtTFB2; h-mtTFB; h-mtTFB2; HCV NS5A transactivated protein 5; HCV NS5A-transactivated protein 5; hepatitis C virus NS5A transactivated protein 5; Hepatitis C virus NS5A-transactivated protein 5; Hkp1; hTFB2M; Mitochondrial 12S rRNA dimethylase 2; mitochondrial; Mitochondrial dimethyladenosine transferase 2; Mitochondrial transcription factor B2; mtTFB; mtTFB2; NS5ATP5; S-adenosylmethionine-6-N''; TFB2M_HUMAN; Transcription Factor B2; Transcription factor B2 mitochondrial; anti-TFB2M antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
TFB2M Antibody detects endogenous levels of total TFB2M.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TFB2M antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
Subunit Structure
Homodimer. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM.
Tissue Specificity
Ubiquitously expressed.
Function
S-adenosyl-L-methionine-dependent rRNA methyltransferase which may methylate two specific adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA (Probable). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Stimulates transcription independently of the methyltransferase activity.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-TFB2M antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,349 Da
NCBI Official Full Name
dimethyladenosine transferase 2, mitochondrial
NCBI Official Synonym Full Names
transcription factor B2, mitochondrial
NCBI Official Symbol
TFB2M
NCBI Official Synonym Symbols
Hkp1; mtTFB2
NCBI Protein Information
dimethyladenosine transferase 2, mitochondrial; HCV NS5A-transactivated protein 5; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2; h-mtTFB; h-mtTFB2; hTFB2M; hepatitis C virus NS5A-transactivated protein 5; mitochondrial 12S rRNA dimet
UniProt Protein Name
Dimethyladenosine transferase 2, mitochondrial
Protein Family
UniProt Gene Name
TFB2M
UniProt Synonym Gene Names
NS5ATP5; HCV NS5A-transactivated protein 5; h-mtTFB; h-mtTFB2; hTFB2M; mtTFB2
UniProt Entry Name
TFB2M_HUMAN
Uniprot Description
TFB2M: S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
Protein type: EC 2.1.1.-; Methyltransferase; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1q44
Cellular Component: mitochondrial matrix
Molecular Function: rRNA (adenine-N6,N6-)-dimethyltransferase activity; transcription cofactor activity
Biological Process: mitochondrion organization and biogenesis; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; rRNA methylation; gene expression; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter
Research Articles on TFB2M
Similar Products
Product Notes
The TFB2M tfb2m (Catalog #AAA9629099) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TFB2M Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TFB2M can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the TFB2M tfb2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFB2M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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