Western Blot (WB)
(Western blot analysis of extracts from various samples, using Nuclear factor 1 C-type Antibody. Lane 1: HepG2 cells(heat shock treatment), blocked with antigen-specific peptides, Lane 2: HepG2 cells(heat shock treatment), Lane 3: P19 cells(serum starvation treatment), Lane 4: VERO cells(H2O2 treatment).)
Rabbit Nuclear factor 1 C-type Polyclonal Antibody | anti-NFIC antibody
Nuclear factor 1 C-type Antibody
ELISA(peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of extracts from various samples, using Nuclear factor 1 C-type Antibody. Lane 1: HepG2 cells(heat shock treatment), blocked with antigen-specific peptides, Lane 2: HepG2 cells(heat shock treatment), Lane 3: P19 cells(serum starvation treatment), Lane 4: VERO cells(H2O2 treatment).)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using Nuclear factor 1 C-type Antibody. Lane 1: HepG2 cells(heat shock treatment), blocked with antigen-specific peptides, Lane 2: HepG2 cells(heat shock treatment), Lane 3: P19 cells(serum starvation treatment), Lane 4: VERO cells(H2O2 treatment).)
Immunohistochemistry (IHC)
(At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using MAP4 Antibody. Lane 1: PC12(H2O2 treatment), blocked with antigen-specific peptides, Lane 2: PC12(H2O2 treatment), Lane 3: Hela cells(heat shock treatment), Lane 4: HepG2 cells(serum starvation treatment).Observed bands: 200kDa)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using MAP4 Antibody. Lane 1: PC12(H2O2 treatment), blocked with antigen-specific peptides, Lane 2: PC12(H2O2 treatment), Lane 3: Hela cells(heat shock treatment), Lane 4: HepG2 cells(serum starvation treatment).Observed bands: 200kDa)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
NFI-C: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleolus; nucleus
Molecular Function: transcription factor activity
Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Research Articles on NFIC
Similar Products
Product Notes
The NFIC nfic (Catalog #AAA9629020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nuclear factor 1 C-type Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclear factor 1 C-type can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA)(peptide). WB: 1:500-1:2000 ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the NFIC nfic for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuclear factor 1 C-type, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
