EEF1D blocking peptide

EEF1D Blocking Peptide

Cat. Num. AAA9626340

• Gene Names: EEF1D; EF1D; EF-1D; FP1047
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: EEF1D; EEF1D Blocking Peptide; Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein); Eukaryotic translation elongation factor 1 delta; FP1047; Guanine nucleotide exchange protein; EEF1D blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for EEF1D blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EEF1D blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 304555583
• NCBI GeneID: 1936
• NCBI Accession #: NP_001123525.2
• NCBI GenBank Nucleotide #: NM_001130053.3
• UniProt Accession #: P29692; Q4VBZ6; Q969J1; Q96I38; B4DDU4; D3DWK3; E9PBQ9
• Molecular Weight: 29,071 Da
• NCBI Official Full Name: elongation factor 1-delta isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• NCBI Official Symbol: EEF1D
• NCBI Official Synonym Symbols: EF1D; EF-1D; FP1047
• NCBI Protein Information: elongation factor 1-delta; EF-1-delta; antigen NY-CO-4; guanine nucleotide exchange protein
• UniProt Protein Name: Elongation factor 1-delta
• Protein Family: Elongation factor
• UniProt Gene Name: EEF1D
• UniProt Synonym Gene Names: EF1D; EF-1-delta
• UniProt Entry Name: EF1D_HUMAN

NCBI Description

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]

Uniprot Description

EEF1D: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Belongs to the EF-1-beta/EF-1-delta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: eukaryotic translation elongation factor 1 complex; endoplasmic reticulum; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: signal transducer activity; protein binding; transcription activator binding; DNA binding; translation factor activity, nucleic acid binding; heat shock protein binding; translation elongation factor activity

Biological Process: cellular protein metabolic process; translational elongation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; translation; transcription, DNA-dependent; mRNA transcription; gene expression; signal transduction