hSET1 Blocking Peptide | SETD1A blocking peptide

hSET1 Blocking Peptide

Cat. Num. AAA9622085

• Gene Names: SETD1A; Set1; KMT2F; Set1A
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: hSET1; hSET1 Blocking Peptide; Histone-lysine N-methyltransferase SETD1A; hSET1A; KMT2F; Lysine N methyltransferase 2F; Lysine N-methyltransferase 2F; SET domain containing 1A; SET domain containing protein 1A; SET domain-containing protein 1A; SET1; Set1 Ash2 histone methyltransferase complex subunit SET1; Set1/Ash2 histone methyltransferase complex subunit SET1; SET1A; SET1A_HUMAN; SETD1A; SETD1A blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for SETD1A blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SETD1A blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 749385041
• NCBI GeneID: 9739
• NCBI Accession #: NM_014712.2
• NCBI GenBank Nucleotide #: NM_014712.2
• UniProt Accession #: O15047; Q6PIF3; Q8TAJ6; A6NP62
• Molecular Weight: ~61 kDa
• NCBI Official Full Name: Homo sapiens SET domain containing 1A (SETD1A), mRNA
• NCBI Official Synonym Full Names: SET domain containing 1A
• NCBI Official Symbol: SETD1A
• NCBI Official Synonym Symbols: Set1; KMT2F; Set1A
• NCBI Protein Information: histone-lysine N-methyltransferase SETD1A
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD1A
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETD1A
• UniProt Synonym Gene Names: hSET1A

NCBI Description

The protein encoded by this gene is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily. [provided by RefSeq, Dec 2016]

Uniprot Description

SETD1A: Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys- 9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: histone methyltransferase complex; nuclear chromatin; nuclear speck; nucleoplasm; nucleus

Molecular Function: beta-catenin binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding; transcription factor binding