DNase II Blocking Peptide | DNASE2 blocking peptide

DNase II Blocking Peptide

Cat. Num. AAA9622062

• Gene Names: DNASE2; DNL; DNL2; DNASE2A
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: DNase II; DNase II Blocking Peptide; Acid DNase; Deoxyribonuclease 2 alpha; Deoxyribonuclease 2; Deoxyribonuclease II alpha; Deoxyribonuclease II; Deoxyribonuclease II lysosomal; Deoxyribonuclease-2-alpha; Deoxyribonuclease2; DeoxyribonucleaseII; DNASE 2; DNASE 2A; DNase II alpha; DNase II lysosomal; DNASE2; DNASE2A; DNaseII; DNL 2; DNL; DNL2; DNS2A_HUMAN; Lysosomal DNase II; R31240 2; R31240_2; DNASE2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for DNASE2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DNASE2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 2921838
• NCBI GeneID: 1777
• UniProt Accession #: O00115; O43910; B2RD06; B7Z4K6
• Molecular Weight: Aprox: 40 kDa
• NCBI Official Full Name: Homo sapiens deoxyribonuclease II alpha mRNA, complete cds
• NCBI Official Synonym Full Names: deoxyribonuclease II, lysosomal
• NCBI Official Symbol: DNASE2
• NCBI Official Synonym Symbols: DNL; DNL2; DNASE2A
• NCBI Protein Information: deoxyribonuclease-2-alpha; R31240_2; acid DNase; DNase II alpha; lysosomal DNase II; DNase II, lysosomal; deoxyribonuclease II alpha
• UniProt Protein Name: Deoxyribonuclease-2-alpha
• Protein Family: Deoxyribonuclease
• UniProt Gene Name: DNASE2
• UniProt Synonym Gene Names: DNASE2A; DNL2; DNase II alpha
• UniProt Entry Name: DNS2A_HUMAN

NCBI Description

This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008]

Uniprot Description

DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family.

Protein type: Deoxyribonuclease; EC 3.1.22.1

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; intracellular

Molecular Function: DNA binding; deoxyribonuclease II activity

Biological Process: erythrocyte differentiation; DNA fragmentation during apoptosis; DNA metabolic process