EIF2B1 blocking peptide

EIF2B1 Blocking Peptide

Cat. Num. AAA9621097

• Gene Names: EIF2B1; EIF2B; EIF2BA
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: EIF2B1; EIF2B1 Blocking Peptide; EI2BA_HUMAN; eIF-2B GDP-GTP exchange factor subunit alpha; EIF2B; Eif2b1; EIF2BA; Eukaryotic translation initiation factor 2B subunit 1 alpha 26kDa; Eukaryotic translation initiation factor 2B subunit alpha; Translation initiation factor eIF-2B subunit alpha; EIF2B1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for EIF2B1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EIF2B1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4503503
• NCBI GeneID: 1967
• NCBI Accession #: NP_001405.1
• NCBI GenBank Nucleotide #: NM_001414.3
• UniProt Accession #: Q14232; Q3SXP4; A6NLY9; B4DGX0
• Molecular Weight: 24,618 Da
• NCBI Official Full Name: translation initiation factor eIF-2B subunit alpha
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
• NCBI Official Symbol: EIF2B1
• NCBI Official Synonym Symbols: EIF2B; EIF2BA
• NCBI Protein Information: translation initiation factor eIF-2B subunit alpha; eIF-2B GDP-GTP exchange factor subunit alpha
• UniProt Protein Name: Translation initiation factor eIF-2B subunit alpha
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF2B1
• UniProt Synonym Gene Names: EIF2BA
• UniProt Entry Name: EI2BA_HUMAN

NCBI Description

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Oct 2009]

Uniprot Description

eIF2B-alpha: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Defects in EIF2B1 are a cause of leukodystrophy with vanishing white matter (VWM). VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Belongs to the eIF-2B alpha/beta/delta subunits family.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: eukaryotic translation initiation factor 2B complex; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; GDP binding; GTP binding; translation initiation factor activity; guanyl-nucleotide exchange factor activity; S-methyl-5-thioribose-1-phosphate isomerase activity; enzyme regulator activity

Biological Process: response to peptide hormone stimulus; translation; cellular response to stimulus; cellular protein metabolic process; methionine salvage; response to heat; translational initiation; response to glucose stimulus; negative regulation of translation initiation in response to stress; gene expression; oligodendrocyte development; regulation of translational initiation; positive regulation of GTPase activity

Disease: Leukoencephalopathy With Vanishing White Matter