DHX9 blocking peptide

DHX9 Blocking Peptide

Cat. Num. AAA9620971

• Gene Names: DHX9; LKP; RHA; DDX9; NDH2; NDHII
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: DHX9; DHX9 Blocking Peptide; ATP dependent RNA helicase A; ATP-dependent RNA helicase A; DDX 9; DDX9; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9; DEAD/H box 9; DEAD/H box polypeptide 9; DEAH (Asp Glu Ala His) box polypeptide 9; DEAH box polypeptide 9; DEAH box protein 9; DHX 9; dhx9; DHX9_HUMAN; Leukophysin; LKP; NDH 2; NDH II; NDH2; NDHII; Nuclear DNA helicase II; RHA; DHX9 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for DHX9 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DHX9 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 100913206
• NCBI GeneID: 1660
• NCBI Accession #: NP_001348.2
• NCBI GenBank Nucleotide #: NM_001357.4
• UniProt Accession #: Q08211; Q05CI5; Q12803; Q32Q22; Q5VY62; Q6PD69; Q99556; B2RNV4
• Molecular Weight: 24,336 Da
• NCBI Official Full Name: Homo sapiens DEAH (Asp-Glu-Ala-His) box helicase 9 (DHX9), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: DEAH (Asp-Glu-Ala-His) box helicase 9
• NCBI Official Symbol: DHX9
• NCBI Official Synonym Symbols: LKP; RHA; DDX9; NDH2; NDHII
• NCBI Protein Information: ATP-dependent RNA helicase A; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; leukophysin; nuclear DNA helicase II
• UniProt Protein Name: ATP-dependent RNA helicase A
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DHX9
• UniProt Synonym Gene Names: DDX9; LKP; NDH2; RHA; LKP; NDH II
• UniProt Entry Name: DHX9_HUMAN

NCBI Description

This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010]

Uniprot Description

DDX9: a helicase that unwinds double-stranded DNA and RNA in a 3' to 5' direction. Interacts with the RNA-induced silencing complex (RISC) in human cells and functions in RISC loading. Generates multiple secondary structures that influence RNA-binding proteins. Has a dsRNA binding motif at the N-terminus, RGG-box at the carboxyl terminus, and a bidirectional nuclear transport domain of 110 amino acids at the carboxyl terminus. May play a role in X-linked gene expression.

Protein type: Nuclear receptor co-regulator; Spliceosome; Nucleolus; Helicase; RNA splicing; EC 3.6.4.13; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: nucleoplasm; centrosome; membrane; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; ATP-dependent RNA helicase activity; ATP binding; RNA helicase activity

Biological Process: osteoblast differentiation; circadian rhythm; RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing; positive regulation of interferon type I production; innate immune response; gene expression; DNA duplex unwinding