ERO1LB blocking peptide

ERO1LB Blocking Peptide

Cat. Num. AAA9620904

• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ERO1LB; ERO1LB Blocking Peptide; Endoplasmic oxidoreductin-1-like protein B; Endoplasmic reticulum oxidoreductin-1-like protein B; ERO1; S. cerevisiae; homolog of; beta; ERO1-L-beta; ERO1-like beta (S. cerevisiae); ERO1-like protein beta; ERO1B_HUMAN; Ero1lb; Oxidoreductin-1-L-beta; ERO1LB blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ERO1LB blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ERO1LB blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 239582761
• NCBI GeneID: 56605
• NCBI Accession #: NP_063944.3
• NCBI GenBank Nucleotide #: NM_019891.3
• UniProt Accession #: Q86YB8; Q5T1H4; Q8IZ11; Q9NR62; B4DF57
• Molecular Weight: 16,096 Da
• NCBI Official Full Name: ERO1-like protein beta
• NCBI Official Synonym Full Names: ERO1-like beta (S. cerevisiae)
• NCBI Official Symbol: ERO1LB
• NCBI Protein Information: ERO1-like protein beta; ERO1-L-beta; oxidoreductin-1-L-beta; endoplasmic oxidoreductin-1-like protein B; endoplasmic reticulum oxidoreductin-1-like protein B
• UniProt Protein Name: ERO1-like protein beta
• UniProt Gene Name: ERO1LB
• UniProt Synonym Gene Names: ERO1-L-beta
• UniProt Entry Name: ERO1B_HUMAN

Uniprot Description

ERO1LB: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress. Belongs to the EROs family.

Protein type: Endoplasmic reticulum; Oxidoreductase; EC 1.8.4.-

Chromosomal Location of Human Ortholog: 1q42.2-q43

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum

Molecular Function: protein binding; unfolded protein binding; protein disulfide isomerase activity; protein disulfide oxidoreductase activity; oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor

Biological Process: insulin processing; protein maturation via protein folding; extracellular matrix organization and biogenesis; 4-hydroxyproline metabolic process; protein folding; cell redox homeostasis; glucose homeostasis