ITPK1 blocking peptide

ITPK1 Blocking Peptide

Cat. Num. AAA9614992

• Gene Names: ITPK1; ITRPK1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ITPK1; ITPK1 Blocking Peptide; 3; 4)P(3) 5/6-kinase; 4-trisphosphate 5/6-kinase; Inositol 1 3 4 triphosphate 5/6 kinase; Inositol 1 3 4 trisphosphate 5/6 kinase; Inositol 1; Inositol tetrakisphosphate 1 kinase; Inositol triphosphate 5/6 kinase; Inositol-tetrakisphosphate 1-kinase; Inositol-triphosphate 5/6-kinase; Ins(1 3 4)P(3) 5/6 kinase; Ins(1; ITPK 1; itpk1; ITPK1_HUMAN; ITRPK 1; ITRPK1; ITPK1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ITPK1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ITPK1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 217272844
• NCBI GeneID: 3705
• NCBI Accession #: NP_001136065.1
• NCBI GenBank Nucleotide #: NM_001142593.2
• UniProt Accession #: Q13572; Q9BTL6; Q9H2E7
• Molecular Weight: 45,621 Da
• NCBI Official Full Name: inositol-tetrakisphosphate 1-kinase isoform a
• NCBI Official Synonym Full Names: inositol-tetrakisphosphate 1-kinase
• NCBI Official Symbol: ITPK1
• NCBI Official Synonym Symbols: ITRPK1
• NCBI Protein Information: inositol-tetrakisphosphate 1-kinase; ins(1,3,4)P(3) 5/6-kinase; inositol-triphosphate 5/6-kinase; inositol 1,3,4-triphosphate 5/6 kinase; inositol 1,3,4-trisphosphate 5/6 kinase; inositol 1,3,4-trisphosphate 5/6-kinase
• UniProt Protein Name: Inositol-tetrakisphosphate 1-kinase
• Protein Family: Inositol-tetrakisphosphate 1-kinase
• UniProt Gene Name: ITPK1
• UniProt Synonym Gene Names: Inositol-triphosphate 5/6-kinase
• UniProt Entry Name: ITPK1_HUMAN

Uniprot Description

ITPK1: Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain. Belongs to the ITPK1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, lipid; EC 2.7.1.134; EC 2.7.1.159; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 14q31

Cellular Component: cytosol

Molecular Function: magnesium ion binding; inositol tetrakisphosphate 1-kinase activity; isomerase activity; catalytic activity; ATP binding

Biological Process: inositol phosphate metabolic process; dephosphorylation; signal transduction; blood coagulation; phosphorylation; inositol trisphosphate metabolic process