DUS2L blocking peptide

DUS2L Blocking Peptide

Cat. Num. AAA9614888

• Gene Names: DUS2L; DUS2; SMM1; URLC8
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: DUS2L; DUS2L Blocking Peptide; 2310016K04Rik; dihydrouridine synthase 2 like; SMM1 homolog (S. cerevisiae); DUS2; FLJ20399; SMM1; tRNA dihydrouridine synthase 2 like; Up regulated in lung cancer; URLC8; DUS2L blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for DUS2L blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DUS2L blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 419635745
• NCBI GeneID: 54920
• NCBI Accession #: NP_001258691.1
• NCBI GenBank Nucleotide #: NM_001271762.1
• UniProt Accession #: Q9NX74; Q4H4D9; A8K3G3
• Molecular Weight: 55,050 Da
• NCBI Official Full Name: tRNA-dihydrouridine(20) synthase
• NCBI Official Synonym Full Names: dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae)
• NCBI Official Symbol: DUS2L
• NCBI Official Synonym Symbols: DUS2; SMM1; URLC8
• NCBI Protein Information: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like; tRNA-dihydrouridine(20) synthase [NAD(P)+]-like; up-regulated in lung cancer protein 8
• UniProt Protein Name: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
• UniProt Gene Name: DUS2L
• UniProt Synonym Gene Names: DUS2; URLC8; hDUS2
• UniProt Entry Name: DUS2L_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

DUS2L: Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Belongs to the dus family. Dus2 subfamily.

Protein type: RNA-binding; Oxidoreductase; EC 1.3.1.-; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: mitochondrion; endoplasmic reticulum; cytoplasm

Molecular Function: protein binding; FAD binding; protein kinase inhibitor activity; double-stranded RNA binding; tRNA dihydrouridine synthase activity

Biological Process: negative regulation of protein kinase activity