ATRX blocking peptide

ATRX Blocking Peptide

Cat. Num. AAA9614876

• Gene Names: ATRX; JMS; XH2; XNP; MRX52; RAD54; RAD54L; ZNF-HX
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ATRX; ATRX Blocking Peptide; Alpha thalassemia/mental retardation syndrome X linked homolog; ATP dependent helicase ATRX; ATP-dependent helicase ATRX; ATR2; Atrx; ATRX_HUMAN; DNA dependent ATPase and helicase; Helicase 2; X linked; MGC2094; MRXHF1; RAD54; RAD54L; SFM1; SHS; Transcriptional regulator ATRX; X linked helicase II; X linked nuclear protein; X-linked helicase II; X-linked nuclear protein; XH2; XNP; Znf HX; Znf-HX; ATRX blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ATRX blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATRX blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 530788276
• NCBI GeneID: 546
• NCBI Accession #: NP_000480.3
• NCBI GenBank Nucleotide #: NM_000489.5
• UniProt Accession #: P46100; P51068; Q15886; Q59FB5; Q59H31; Q5H9A2; Q5JWI4; Q7Z2J1; Q9H0Z1; Q9NTS3; D3DTE2
• Molecular Weight: 151,556 Da
• NCBI Official Full Name: transcriptional regulator ATRX isoform 1
• NCBI Official Synonym Full Names: ATRX, chromatin remodeler
• NCBI Official Symbol: ATRX
• NCBI Official Synonym Symbols: JMS; XH2; XNP; MRX52; RAD54; RAD54L; ZNF-HX
• NCBI Protein Information: transcriptional regulator ATRX
• UniProt Protein Name: Transcriptional regulator ATRX
• Protein Family: Transcriptional regulator
• UniProt Gene Name: ATRX
• UniProt Synonym Gene Names: RAD54L; XH2; XNP
• UniProt Entry Name: ATRX_HUMAN

NCBI Description

The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2017]

Uniprot Description

ATRX: a global transcriptional regulator. Belongs to the SNF2 family of proteins, many of which modify gene expression via chromatin remodeling activity. Involved in the developmental silencing of imprinted genes in the brain. Contains one PxVxL motif, which is required for interaction with chromoshadow domains. This motif requires additional residues at -7, -6, +4 and +5 relative to the central V which contact the chromoshadow domain. Constitutive mutations in ATRX are associated with brain, facial, and genital abnormalities, and alpha thalassemia. Acquired mutations in ATRX have been observed in preleukemic conditions. Six alternatively spliced human isoforms have been described.

Protein type: DNA repair, damage; Ubiquitin conjugating system; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: Xq21.1

Cellular Component: nucleoplasm; PML body; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA helicase activity; DNA binding; histone binding; zinc ion binding; chromatin binding; helicase activity; DNA translocase activity; methylated histone residue binding; ATP binding

Biological Process: transcription, DNA-dependent; DNA damage response, signal transduction by p53 class mediator; positive regulation of telomere maintenance; DNA repair; Sertoli cell development; DNA duplex unwinding; replication fork processing; DNA recombination; chromatin remodeling; nucleosome assembly; DNA replication-independent nucleosome assembly; regulation of transcription, DNA-dependent; forebrain development; DNA methylation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter

Disease: Alpha-thalassemia/mental Retardation Syndrome, X-linked; Mental Retardation-hypotonic Facies Syndrome, X-linked, 1; Alpha-thalassemia Myelodysplasia Syndrome