Western Blot (WB)
(Western blot analysis of extracts from various samples, using Phospho-PAK4/5/6 (Ser474) Antibody.Lane 1: VERO cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: VERO cells(heat shock treatment),Lane 3: Mouse heart.Observed bands: 72kDa)
Rabbit PAK4/5/6 Polyclonal Antibody | anti-PAK4/5/6 antibody
Phospho-PAK4/5/6 (Ser474) Antibody
Predicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Tissue Specificity: Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
Note: Cotranslocates into nucleus with AR in response to androgen induction.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using Phospho-PAK4/5/6 (Ser474) Antibody.Lane 1: VERO cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: VERO cells(heat shock treatment),Lane 3: Mouse heart.Observed bands: 72kDa)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using Phospho-PAK4/5/6 (Ser474) Antibody.Lane 1: VERO cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: VERO cells(heat shock treatment),Lane 3: Mouse heart.Observed bands: 72kDa)
Western Blot (WB)
(Western blot analysis of PAK4/5/6 (Phospho-Ser474) using TNF treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of PAK4/5/6 (Phospho-Ser474) using TNF treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Immunohistochemistry (IHC)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.1; STE group; STE20 family; PAKB subfamily
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: Golgi apparatus; focal adhesion
Molecular Function: protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: cell proliferation; cell migration; apoptosis; cytoskeleton organization and biogenesis; mitotic cell cycle; cell motility; cell growth; signal transduction; protein amino acid phosphorylation
Research Articles on PAK4/5/6
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Product Notes
The PAK4/5/6 pak4 (Catalog #AAA9614028) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PAK4/5/6 (Ser474) Antibody reacts with Human, Mouse, Rat, Monkey Predicted Reactivity: Pig (100%), Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's PAK4/5/6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:1000-3000 IHC: 1:50-1:200 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PAK4/5/6 pak4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAK4/5/6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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