Rabbit MAP3K8/COT Polyclonal Antibody | anti-MAP3K8/COT antibody

MAP3K8/COT Antibody

Cat. Num. AAA9613691

• Gene Names: MAP3K8; COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: MAP3K8/COT; Polyclonal Antibody; MAP3K8/COT Antibody; AURA2; c COT; Cancer Osaka thyroid oncogene; CCOT; COT; COT proto oncogene serine/threonine protein kinase; EST; ESTF; Ewing sarcoma transformant; FLJ10486; M3K8_HUMAN; MAP3K 8; MAP3K8; MEKK8; Mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Proto oncogene cCot; Proto-oncogene c-Cot; Serine/threonine protein kinase cot; Serine/threonine-protein kinase cot; TPL 2; TPL-2; TPL2; Tumor progression locus 2; anti-MAP3K8/COT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MAP3K8/COT Antibody detects endogenous levels of total MAP3K8/COT.; Tissue Specificity: Expressed in several normal tissues and human tumor-derived cell lines.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MAP3K8/COT antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human MAP3K8/COT.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Phosphorylated on Thr-290; the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Phosphorylated on Ser-400 by IKBKB; the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages.
• Subunit Structure: Forms a ternary complex with NFKB1/p105 and TNIP2. Interacts with NFKB1; the interaction increases the stability of MAP3K8 but inhibits its MEK phosphorylation activity, whereas loss of interaction following LPS stimulation leads to its degradation. Interacts with CD40 and TRAF6; the interaction is required for ERK activation. Interacts with KSR2; the interaction inhibits ERK and NF-kappa-B activation.
• Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
• Subcellular Location: Cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-MAP3K8/COT antibody

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

NCBI and Uniprot Product Information

• NCBI GI #: 346644795
• NCBI GeneID: 1326
• NCBI Accession #: NP_001231063.1
• NCBI GenBank Nucleotide #: NM_001244134.1
• UniProt Accession #: P41279; Q14275; Q5T855; Q9HC81; A8K2Q5; D3DRX1
• Molecular Weight: 52,925 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 8
• NCBI Official Symbol: MAP3K8
• NCBI Official Synonym Symbols: COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 8; proto-oncogene c-Cot; tumor progression locus 2; Ewing sarcoma transformant; cot (cancer Osaka thyroid) oncogene; proto-oncogene serine/threoine protein kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 8
• UniProt Gene Name: MAP3K8
• UniProt Synonym Gene Names: COT; ESTF; TPL-2
• UniProt Entry Name: M3K8_HUMAN

NCBI Description

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

Cot: an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Oncoprotein; EC 2.7.11.25; STE group; STE-Unique family

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: cytoplasm; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of MAPKK activity; apoptosis; regulation of cell differentiation; T cell costimulation; MAPKKK cascade; cell cycle; protein amino acid phosphorylation

Disease: Lung Cancer