Western Blot (WB)
(Western blot analysis of extracts from Jurkat, using NAKED2 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit NAKED2 Polyclonal Antibody | anti-NAKED2 antibody
NAKED2 Antibody
Gene Names
NKD2; Naked2
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Zebrafish (100%), Horse (100%), Dog (100%)
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
NAKED2; Polyclonal Antibody; NAKED2 Antibody; hNkd2; Naked 2; Naked-2; nkd2; NKD2_HUMAN; Protein naked cuticle homolog 2; anti-NAKED2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Horse (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Horse (100%), Dog (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
NAKED2 Antibody detects endogenous levels of total NAKED2.
Tissue Specificity: Expressed in kidney, lung, pancreas and spleen.
Tissue Specificity: Expressed in kidney, lung, pancreas and spleen.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NAKED2 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human NAKED2, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.
Subunit Structure
Interacts with DVL1, DVL2, DVL3 and PPP2R3A (By similarity). Interacts with RNF25 and TGFA (via cytoplasmic domain).
Similarity
The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.Belongs to the NKD family.
Subcellular Location
Cell membrane. Cytoplasm. Cytoplasmic vesicle.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Jurkat, using NAKED2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat, using NAKED2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NAKED2 antibody
Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,314 Da
NCBI Official Full Name
protein naked cuticle homolog 2 isoform 2
NCBI Official Synonym Full Names
naked cuticle homolog 2
NCBI Official Symbol
NKD2
NCBI Official Synonym Symbols
Naked2
NCBI Protein Information
protein naked cuticle homolog 2
UniProt Protein Name
Protein naked cuticle homolog 2
UniProt Gene Name
NKD2
UniProt Synonym Gene Names
Naked-2; hNkd2
NCBI Description
This gene encodes a member of a family of proteins that function as negative regulators of Wnt receptor signaling through interaction with Dishevelled family members. The encoded protein participates in the delivery of transforming growth factor alpha-containing vesicles to the cell membrane. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
Uniprot Description
Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
Research Articles on NAKED2
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Product Notes
The NAKED2 nkd2 (Catalog #AAA9613355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NAKED2 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Zebrafish (100%), Horse (100%), Dog (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's NAKED2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the NAKED2 nkd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAKED2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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