Rabbit RYR1 Polyclonal Antibody | anti-RYR1 antibody

Phospho-RYR1 (Ser2843) Antibody

Cat. Num. AAA9612771

• Gene Names: RYR1; CCO; MHS; RYR; MHS1; RYDR; SKRR; RYR-1; PPP1R137
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: RYR1; Polyclonal Antibody; Phospho-RYR1 (Ser2843) Antibody; CCO; Central core disease of muscle; MHS; MHS1; PPP1R137; Protein phosphatase 1 regulatory subunit 137; Ryanodine receptor 1; RYDR; RYR; RYR-1; RyR1; RYR1_HUMAN; sarcoplasmic reticulum calcium release channel; Skeletal muscle calcium release channel; Skeletal muscle ryanodine receptor; Skeletal muscle-type ryanodine receptor; SKRR; Type 1 ryanodine receptor; anti-RYR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-RYR1 (Ser2843) Antibody detects endogenous levels of RYR1 only when phosphorylated at Ser2843.; Tissue Specificity: Skeletal muscle and brain (cerebellum and hippocampus).
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RYR1 antibody: Immunohistochemistry (IHC), Peptide ELISA (EIA)
• Application Notes: IHC: 1:50-1:200; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human RYR1 around the phosphorylation site of Ser2843.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843. Activated by reversible S-nitrosylation (By similarity). Repeated very high-level exercise increases S-nitrosylation.
• Subunit Structure: Homotetramer. Can also form heterotetramers with RYR2 (By similarity). Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Repeated very high-level exercise decreases interaction with PDE4D and protein phosphatase 1 (PP1). Interacts with CALM; CALM with bound calcium inhibits the RYR1 channel activity. Interacts with S100A1. Interacts with FKBP1A; this stabilizes the closed conformation of the channel. Interacts with CACNA1S; interaction with CACNA1S is important for activation of the RYR1 channel. Interacts with CACNB1. Interacts with TRDN and ASPH; these interactions stimulate RYR1 channel activity. Interacts with SELENON (By similarity). Interacts with scorpion calcins (AC P0DPT1; AC P0DM30; AC A0A1L4BJ42; AC P59868; AC P60254; AC B8QG00; AC L0GBR1; AC P60252; AC P60253) (By similarity).
• Similarity: The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum (SR) and the T-tubule. Pore opening is mediated via the cytoplasmic calcium-binding domains that mediate a small rotation of the channel-forming transmembrane regions that then leads to channel opening.Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.
• Subcellular Location: Sarcoplasmic reticulum membrane>Multi-pass membrane protein. Sarcoplasmic reticulum.; Note: The number of predicted transmembrane domains varies between orthologs, but the 3D-structures show the presence of six transmembrane regions. Both N-terminus and C-terminus are cytoplasmic.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(At 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Mouse skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-RYR1 antibody

Calcium channel that mediates the release of Ca (2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca (2+) leaking into the cytoplasm. Can also mediate the release of Ca (2+) from intracellular stores in neurons, and may thereby promote prolonged Ca (2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 108935904
• NCBI GeneID: 6261
• UniProt Accession #: P21817; Q16314; Q16368; Q9NPK1; Q9P1U4
• Molecular Weight: 565,176 Da
• NCBI Official Full Name: Ryanodine receptor 1
• NCBI Official Synonym Full Names: ryanodine receptor 1 (skeletal)
• NCBI Official Symbol: RYR1
• NCBI Official Synonym Symbols: CCO; MHS; RYR; MHS1; RYDR; SKRR; RYR-1; PPP1R137
• NCBI Protein Information: ryanodine receptor 1; central core disease of muscle; type 1-like ryanodine receptor; skeletal muscle ryanodine receptor; skeletal muscle calcium release channel; protein phosphatase 1, regulatory subunit 137; sarcoplasmic reticulum calcium release channe
• UniProt Protein Name: Ryanodine receptor 1
• Protein Family: Ryanodine receptor
• UniProt Gene Name: RYR1
• UniProt Synonym Gene Names: RYDR; RYR-1; RyR1
• UniProt Entry Name: RYR1_HUMAN

NCBI Description

This gene encodes a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

RYR1: Calcium channel that controls communication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Can mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal development of muscle fibers, skeletal muscle, heart morphogenesis, and skin development and ossification during embryogenesis. Defects in RYR1 are the cause of malignant hyperthermia susceptibility type 1 (MHS1) and central core disease of muscle (CCD). CCD is an autosomal dominant congenital myopathy, but a severe autosomal recessive form also exists. Defects in RYR1 are the cause of multiminicore disease with external ophthalmoplegia (MMDO), congenital myopathy with fiber-type disproportion (CFTD). Defects in RYR1 may be a cause of Samaritan myopathy, a congenital myopathy with benign course. Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass; Channel, calcium

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: I band; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; sarcoplasmic reticulum; integral to plasma membrane; cytoplasm; junctional membrane complex; T-tubule; plasma membrane; cell cortex

Molecular Function: voltage-gated calcium channel activity; calmodulin binding; protein binding; protease binding; calcium-release channel activity; calcium channel activity; ryanodine-sensitive calcium-release channel activity

Biological Process: skin development; cytosolic calcium ion homeostasis; muscle contraction; calcium ion transport; release of sequestered calcium ion into cytosol; response to hypoxia; skeletal muscle fiber development; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; transmembrane transport; response to caffeine

Disease: Minicore Myopathy With External Ophthalmoplegia; Malignant Hyperthermia, Susceptibility To, 1; Central Core Disease Of Muscle; Myopathy, Congenital, With Fiber-type Disproportion