Western Blot (WB)
(Western blot analysis of extracts from Hek293Â , using Thymosin beta 10 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit Thymosin beta 10 Polyclonal Antibody | anti-TMSB10 antibody
Thymosin beta 10 Antibody
Gene Names
TMSB10; TB10; MIG12
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
Peptide affinity purification
Synonyms
Thymosin beta 10; Polyclonal Antibody; Thymosin beta 10 Antibody; MIG 12; MIG12; Migration inducing gene 12; Migration inducing protein 12; Ptmb10; TB 10; TB10; Thyb 10; Thyb10; Thymosin beta-10; Tmsb10; TYB10_HUMAN; anti-TMSB10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Thymosin beta 10 Antibody detects endogenous levels of total Thymosin beta 10.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
44
Applicable Applications for anti-TMSB10 antibody
ELISA (EIA)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:500-1:2000
ELISA(peptide): 1:20000-1:40000
The optimal dilutions should be determined by the end user.
Important Note: For western blots , incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night.
ICC: 1:100-1:500
WB: 1:500-1:2000
ELISA(peptide): 1:20000-1:40000
The optimal dilutions should be determined by the end user.
Important Note: For western blots , incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night.
Immunogen
A synthesized peptide derived from human Thymosin beta 10, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Hek293Â , using Thymosin beta 10 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hek293Â , using Thymosin beta 10 Antibody. The lane on the left was treated with blocking peptide.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612495 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612495 1:200) and mouse anti-beta tubulin Ab (MBS96103287 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612495 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612495 1:200) and mouse anti-beta tubulin Ab (MBS96103287 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Related Product Information for anti-TMSB10 antibody
Function: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
5kDa
NCBI Official Full Name
thymosin beta-10
NCBI Official Synonym Full Names
thymosin beta 10
NCBI Official Symbol
TMSB10
NCBI Official Synonym Symbols
TB10; MIG12
NCBI Protein Information
thymosin beta-10
UniProt Protein Name
Thymosin beta-10
Protein Family
UniProt Gene Name
TMSB10
UniProt Synonym Gene Names
PTMB10; THYB10
UniProt Entry Name
TYB10_HUMAN
Uniprot Description
TMSB10: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: cytoskeleton; cytoplasm
Molecular Function: actin monomer binding
Biological Process: sequestering of actin monomers; actin filament organization
Research Articles on TMSB10
Similar Products
Product Notes
The TMSB10 tmsb10 (Catalog #AAA9612495) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Thymosin beta 10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Thymosin beta 10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). IF: 1:100-1:500 ICC: 1:100-1:500 WB: 1:500-1:2000 ELISA(peptide): 1:20000-1:40000 The optimal dilutions should be determined by the end user. Important Note: For western blots, incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night. Researchers should empirically determine the suitability of the TMSB10 tmsb10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thymosin beta 10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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