Western Blot (WB)
(Western blot analysis of extracts from B16F10 cells, using SNRP70 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit SNRP70 Polyclonal Antibody | anti-SNRNP70 antibody
SNRP70 Antibody
Gene Names
SNRNP70; RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
Reactivity
Human, Mouse, RatPredicted Reactivity: Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SNRP70; Polyclonal Antibody; SNRP70 Antibody; RNPU1Z; Rnulp70; RPU1; Small nuclear ribonucleoprotein 70 (U1); Small nuclear ribonucleoprotein 70kD polypeptide (RNP antigen); Small nuclear ribonucleoprotein 70kDa (U1); Small nuclear ribonucleoprotein 70kDa polypeptide (RNP antigen); snRNP70; Snrp 70; U1 70K; U1 small nuclear ribonucleoprotein 70 kDa; U1 small nuclear ribonucleoprotein polypeptide A; U1 snRNP 70 kDa; U170K; U1AP; U1AP1; U1RNP; anti-SNRNP70 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(100%)
Predicted Reactivity: Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
SNRP70 Antibody detects endogenous levels of total SNRP70.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
428
Applicable Applications for anti-SNRNP70 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF: 1:100-1:500
ICC: 1:100-1:500
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
IF: 1:100-1:500
ICC: 1:100-1:500
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human SNRP70, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from B16F10 cells, using SNRP70 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from B16F10 cells, using SNRP70 Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612309 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612309 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612309 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612309) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612309 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612309) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Related Product Information for anti-SNRNP70 antibody
Function: Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome (PubMed:19325628, PubMed:25555158). SNRNP70 binds to the loop I region of U1-snRNA (PubMed:2467746, PubMed:19325628, PubMed:25555158).Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.
Post Translational Modifications: The N-terminus is blocked.Extensively phosphorylated on serine residues in the C-terminal region.
Subcellular Location: Nucleus speckle. Nucleus>Nucleoplasm. Note: Colocalizes with SCNM1 and LUC7L2 in nuclear speckles.
Subunit Structure: Component of the U1 snRNP (PubMed:19325628, PubMed:21113136, PubMed:25555158). The U1 snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C (PubMed:19325628, PubMed:21113136, PubMed:25555158). Interacts with SCNM1 (By similarity). Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2 (PubMed:9531537). Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10 (PubMed:10339552). Interacts with dephosphorylated SFRS13A and SFPQ (PubMed:11514619, PubMed:14765198). Interacts with NUDT21/CPSF5, CPSF6, SCAF11, and ZRANB2 (PubMed:14561889, PubMed:11448987, PubMed:9447963). Interacts with GEMIN5 (PubMed:25911097). Interacts with FUS.
Similarity: The RRM domain mediates interaction with U1 RNA.
Post Translational Modifications: The N-terminus is blocked.Extensively phosphorylated on serine residues in the C-terminal region.
Subcellular Location: Nucleus speckle. Nucleus>Nucleoplasm. Note: Colocalizes with SCNM1 and LUC7L2 in nuclear speckles.
Subunit Structure: Component of the U1 snRNP (PubMed:19325628, PubMed:21113136, PubMed:25555158). The U1 snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C (PubMed:19325628, PubMed:21113136, PubMed:25555158). Interacts with SCNM1 (By similarity). Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2 (PubMed:9531537). Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10 (PubMed:10339552). Interacts with dephosphorylated SFRS13A and SFPQ (PubMed:11514619, PubMed:14765198). Interacts with NUDT21/CPSF5, CPSF6, SCAF11, and ZRANB2 (PubMed:14561889, PubMed:11448987, PubMed:9447963). Interacts with GEMIN5 (PubMed:25911097). Interacts with FUS.
Similarity: The RRM domain mediates interaction with U1 RNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)51kD.
Predicted Molecular Weight: (Calculated)52kDa.
Predicted Molecular Weight: (Calculated)52kDa.
NCBI Official Full Name
U1 small nuclear ribonucleoprotein 70 kDa isoform 2
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein U1 subunit 70
NCBI Official Symbol
SNRNP70
NCBI Official Synonym Symbols
RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
NCBI Protein Information
U1 small nuclear ribonucleoprotein 70 kDa
UniProt Protein Name
U1 small nuclear ribonucleoprotein 70 kDa
Protein Family
UniProt Gene Name
SNRNP70
UniProt Synonym Gene Names
RNPU1Z; RPU1; SNRP70; U1AP1; U1 snRNP 70 kDa; U1-70K; snRNP70
UniProt Entry Name
RU17_HUMAN
Uniprot Description
snRNP 70: mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. Participates in pre-mRNA splicing and exonic splicing complexes. A major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus. Four alternatively spliced human isoforms have been described. The truncated isoforms (2-4) cannot bind U1-snRNA.
Protein type: RNA-binding; RNA splicing; Spliceosome
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; spliceosome; nuclear speck; snRNP U1; nucleus
Molecular Function: protein binding; RNA binding; nucleotide binding
Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; regulation of RNA splicing; RNA splicing; gene expression; mRNA processing
Research Articles on SNRNP70
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Product Notes
The SNRNP70 snrnp70 (Catalog #AAA9612309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SNRP70 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's SNRP70 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:500 ICC: 1:100-1:500 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SNRNP70 snrnp70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNRP70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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