Rabbit Transportin 1 Polyclonal Antibody | anti-TNPO1 antibody

Transportin 1 Antibody

Cat. Num. AAA9612050

• Gene Names: TNPO1; MIP; TRN; IPO2; MIP1; KPNB2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Transportin 1; Polyclonal Antibody; Transportin 1 Antibody; Importin 2; Importin beta 2; Importin beta-2; IPO 2; IPO2; Karyopherin (importin) beta 2; Karyopherin beta 2; Karyopherin beta-2; KPN B2; KPNB 2; KPNB2; M9 interacting protein; M9 region interacting protein; M9 region interaction protein; MIP 1; MIP; MIP1; OTTHUMP00000126960; OTTHUMP00000222024; OTTHUMP00000226224; TNPO 1; Tnpo1; TNPO1_HUMAN; Transportin; Transportin-1; Transportin1; TRN; anti-TNPO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Transportin 1 Antibody detects endogenous levels of total Transportin 1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 898

• Applicable Applications for anti-TNPO1 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Transportin 1, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using Transportin 1 Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612050 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612050) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-TNPO1 antibody

Function: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:24753571). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19 (By similarity). Mediates nuclear import of ADAR/ADAR1 isoform 1 and isoform 5 in a RanGTP-dependent manner (PubMed:19124606, PubMed:24753571).(Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.

Subcellular Location: Cytoplasm. Nucleus.

Subunit Structure: Identified in a complex that contains TNPO1, RAN and RANBP1 (PubMed:9428644). Binds HNRPA1, HNRPA2, HNRNPDL, RPL23A, RPS7, RPL5, RAN and SRP19. Interacts with H2A, H2B, H3 and H4 histones (By similarity). Interacts with isoform 1 and isoform 5 of ADAR/ADAR1 (via DRBM 3 domain) (PubMed:19124606, PubMed:24753571). Interacts with SNAI1 (via zinc fingers); the interaction mediates SNAI1 nuclear import (PubMed:19386897). Interacts with SNAI2 (via zinc fingers) (PubMed:19386897).(Microbial infection) Binds to HIV-1 Rev.

Similarity: Belongs to the importin beta family. Importin beta-2 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 133925811
• NCBI GeneID: 3842
• NCBI Accession #: NP_002261.3
• NCBI GenBank Nucleotide #: NP_002261.3
• UniProt Accession #: Q92973
• Molecular Weight: Observed Molecular Weight: (Observed)102kD.; Predicted Molecular Weight: (Calculated)102kDa.
• NCBI Official Full Name: transportin-1 isoform 1
• NCBI Official Synonym Full Names: transportin 1
• NCBI Official Symbol: TNPO1
• NCBI Official Synonym Symbols: MIP; TRN; IPO2; MIP1; KPNB2
• NCBI Protein Information: transportin-1
• UniProt Protein Name: Transportin-1
• UniProt Gene Name: TNPO1
• UniProt Synonym Gene Names: KPNB2; MIP1; TRN; MIP
• UniProt Entry Name: TNPO1_HUMAN

NCBI Description

This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in several transcript variants encoding different proteins. [provided by RefSeq, Jun 2018]

Uniprot Description

KPNB2: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in nuclear import of M9- containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Belongs to the importin beta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; nuclear localization sequence binding; Ran GTPase binding

Biological Process: viral reproduction; protein import into nucleus, translocation; organelle organization and biogenesis; gene expression