Rabbit ATG3 Polyclonal Antibody | anti-ATG3 antibody

ATG3 Antibody

Cat. Num. AAA9610875

• Gene Names: ATG3; APG3; APG3L; PC3-96; APG3-LIKE
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ATG3; Polyclonal Antibody; ATG3 Antibody; 2610016C12Rik; Apg 3; APG3; APG3 autophagy 3 like; APG3 like; S. cerevisiae; homolog of; APG3-like; APG3L; Apg3p; ATG 3; ATG3 autophagy related 3 homolog; ATG3 autophagy related 3 homolog (S. cerevisiae); ATG3_HUMAN; Autophagy 3; Autophagy Apg3p/Aut1p like; autophagy related 3; Autophagy related protein 3; Autophagy-related protein 3; DKFZp564M1178; FLJ22125; hApg3; MGC15201; OTTHUMP00000214547; OTTHUMP00000214548; PC3 96; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3; anti-ATG3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATG3 Antibody detects endogenous levels of ATG3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 314

• Applicable Applications for anti-ATG3 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ATG3.
• Tissue Specificity: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
• Subcellular Location: Cytoplasm
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of ATG3 in lysates of Jurkat , using ATG3 Antibody.)

Related Product Information for anti-ATG3 antibody

Description: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Post Translational Modifications: Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy. Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis.

Subunit Structure: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Interacts with FNBP1L.

Similarity: Belongs to the ATG3 family.

NCBI and Uniprot Product Information

• NCBI GI #: 19526773
• NCBI GeneID: 64422
• NCBI Accession #: NP_071933
• NCBI GenBank Nucleotide #: NP_071933.2
• UniProt Accession #: Q9NT62
• Molecular Weight: Observed: 40 kDa; Predicted: 36 kDa
• NCBI Official Full Name: ubiquitin-like-conjugating enzyme ATG3 isoform 1
• NCBI Official Synonym Full Names: autophagy related 3
• NCBI Official Symbol: ATG3
• NCBI Official Synonym Symbols: APG3; APG3L; PC3-96; APG3-LIKE
• NCBI Protein Information: ubiquitin-like-conjugating enzyme ATG3
• UniProt Protein Name: Ubiquitin-like-conjugating enzyme ATG3
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG3
• UniProt Synonym Gene Names: APG3; APG3L; APG3-like; hApg3
• UniProt Entry Name: ATG3_HUMAN

NCBI Description

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

ATG3: an E2-like enzyme essential for Apg8p lipidation.

Protein type: Enzyme, cellular metabolism; EC 6.3.2.-; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol

Molecular Function: protein binding; enzyme binding; small conjugating protein ligase activity; APG8 conjugating enzyme activity; APG12 conjugating enzyme activity

Biological Process: mitochondrion degradation; protein ubiquitination; protein modification process; mitochondrial fragmentation during apoptosis; protein targeting to membrane; autophagic vacuole formation