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Immunohistochemistry (IHC) (At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Rabbit BACH1 Polyclonal Antibody | anti-BACH1 antibody

Phospho-BACH1 (Ssr445) Antibody

Gene Names
BACH1; BACH-1; BTBD24
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
BACH1; Polyclonal Antibody; Phospho-BACH1 (Ssr445) Antibody; BACH 1; BACH 1.3; Bach1; BACH1_HUMAN; Basic leucine zipper transcription factor 1; basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homolog 1; BTB and CNC homology 1; basic leucine zipper transcription factor 1; HA2303; OTTHUMP00000096563; OTTHUMP00000096564; Transcription regulator protein BACH1; anti-BACH1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-BACH1(Ssr445) Antibody detects endogenous levels of BACH1 only when phosphorylated at Ssr445.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
736
Applicable Applications for anti-BACH1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human BACH1 around the phosphorylation site of Ssr445.
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemistry (IHC) (At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemmistry (IHC)

(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemmistry (IHC) (At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemistry (IHC)

(At 1/100 staining mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemistry (IHC) (At 1/100 staining mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Western Blot (WB)

(Western blot analysis of Phospho-BACH1(Ssr445) in lysates of HeLa, using Phospho-BACH1(Ssr445) Antibody.)

Western Blot (WB) (Western blot analysis of Phospho-BACH1(Ssr445) in lysates of HeLa, using Phospho-BACH1(Ssr445) Antibody.)
Related Product Information for anti-BACH1 antibody
Description: Transcriptional regulator that acts as repressor or activator. Binds, in vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK.

Similarity: Belongs to the bZIP family. CNC subfamily.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
571
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 105 kDa
Predicted: 82 kDa
NCBI Official Full Name
transcription regulator protein BACH1
NCBI Official Synonym Full Names
BTB domain and CNC homolog 1
NCBI Official Symbol
BACH1
NCBI Official Synonym Symbols
BACH-1; BTBD24
NCBI Protein Information
transcription regulator protein BACH1
UniProt Protein Name
Transcription regulator protein BACH1
UniProt Gene Name
BACH1
UniProt Entry Name
BACH1_HUMAN

NCBI Description

This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, May 2009]

Uniprot Description

BACH1: a transcriptional regulator that acts as a repressor or activator. Binds, in-vitro, to NF-E2 binding sites on DNA. Plays an important role in coordinating transcription activation and repression by NF-E2. Belongs to the bZIP family, CNC subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: heme binding; transcription factor activity

Biological Process: G1/S-specific transcription in mitotic cell cycle; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; G2/M-specific transcription in mitotic cell cycle

Research Articles on BACH1

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Product Notes

The BACH1 bach1 (Catalog #AAA9610451) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-BACH1 (Ssr445) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's BACH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the BACH1 bach1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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