Rabbit MARCH1 Polyclonal Antibody | anti-MARCH1 antibody

MARCH1 Antibody

Cat. Num. AAA9609798

• Gene Names: MARCH1; RNF171; MARCH-I
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: MARCH1; Polyclonal Antibody; MARCH1 Antibody; DKFZp564M1682; E3 ubiquitin protein ligase MARCH1; E3 ubiquitin-protein ligase MARCH1; FLJ20668; MARCH 1; MARCH-I; March1; MARH1_HUMAN; Membrane associated RING CH protein I; Membrane associated ring finger (C3HC4) 1; E3 ubiquitin protein ligase; Membrane associated RING finger protein 1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; RING finger protein 171; RNF171; anti-MARCH1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MARCH1 antibody detects endogenous levels of total 42430
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 289

• Applicable Applications for anti-MARCH1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human MARCH1
• Subcellular Location: Lysosome; Endosome; Golgi Apparatus; Plasma Membrane
• Tissue Specificity: Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung. Expression is high in follicular B-cells, moderate in dendritic cells and low in splenic T-cells.
• Predicted Cross Reactivity: Rabbit, Chicken
• Similarity: Rabbit (100%), Chicken (86%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from rat muscle, mouse brain, using MARCH1 Antibody.)

Western Blot (WB)

(Western blot analysis of MARCH1 using LOVO whole cell lysates)

Related Product Information for anti-MARCH1 antibody

Function: E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.
Post-translational Modifications: Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins (By similarity).
Similarity: The RING-CH-type zinc finger domain is required for E3 ligase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 261823980
• NCBI GeneID: 55016
• NCBI Accession #: NP_001159845.1
• NCBI GenBank Nucleotide #: NM_001166373.1
• UniProt Accession #: Q8TCQ1; Q9NWR0; D3DP29
• Molecular Weight: Observed: 32 kDa; Predicted: 33 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH1 isoform 1
• NCBI Official Synonym Full Names: membrane associated ring-CH-type finger 1
• NCBI Official Symbol: MARCH1
• NCBI Official Synonym Symbols: RNF171; MARCH-I
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH1
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH1
• UniProt Gene Name: MARCH1
• UniProt Synonym Gene Names: RNF171; MARCH-I

NCBI Description

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).[supplied by OMIM, Mar 2010]

Uniprot Description

E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.