Western Blot (WB)
(Western blot analysis of WNK2 using Jurkat whole cell lysates)
Rabbit anti-Human, Mouse WNK2 Polyclonal Antibody | anti-WNK2 antibody
WNK2 Antibody
Gene Names
WNK2; PRKWNK2; NY-CO-43; SDCCAG43; P/OKcl.13
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
WNK2; Polyclonal Antibody; WNK2 Antibody; Antigen NY-CO-43; KIAA1760; mitogen-activated protein kinase kinase kinase; NY CO 43; P/OKcl.13; PRKWNK2; Protein kinase lysine deficient 2; Protein kinase with no lysine 2; SDCCAG43; Serine/Threonine protein kinase WNK2; Serologically defined colon cancer antigen 43; WNK lysine deficient protein kinase 2; anti-WNK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
WNK2 antibody detects endogenous levels of total WNK2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
2297
Applicable Applications for anti-WNK2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human WNK2
Subcellular Location
Cell Membrane and Cytoplasmic
Tissue Specificity
Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain, skeletal muscle and colon.
Predicted Cross Reactivity
Pig, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Horse (100%), Sheep (91%), Rabbit (100%), Dog (100%), Chicken (89%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of WNK2 using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of WNK2 using Jurkat whole cell lysates)
Related Product Information for anti-WNK2 antibody
Function: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.
Subunit Structure: Forms a complex with the phosphorylated form of STK39.
Post-translational Modifications: Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Subunit Structure: Forms a complex with the phosphorylated form of STK39.
Post-translational Modifications: Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 243 kD
Predicted: 243 kDa
Predicted: 243 kDa
NCBI Official Full Name
serine/threonine-protein kinase WNK2 isoform 1
NCBI Official Synonym Full Names
WNK lysine deficient protein kinase 2
NCBI Official Symbol
WNK2
NCBI Official Synonym Symbols
PRKWNK2; NY-CO-43; SDCCAG43; P/OKcl.13
NCBI Protein Information
serine/threonine-protein kinase WNK2
UniProt Protein Name
Serine/threonine-protein kinase WNK2
Protein Family
UniProt Gene Name
WNK2
UniProt Synonym Gene Names
KIAA1760ImportedManual assertion inferred from database entriesiEMBL:BAB21851.2; PRKWNK2ImportedManual assertion inferred from database entriesiHGNC:14542; SDCCAG43ImportedManual assertion inferred from database entriesiHGNC:14542
NCBI Description
The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Uniprot Description
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.
Research Articles on WNK2
Similar Products
Product Notes
The WNK2 wnk2 (Catalog #AAA9609211) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WNK2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's WNK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the WNK2 wnk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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