Rabbit BCL10 Polyclonal Antibody | anti-BCL10 antibody

BCL10 Antibody

Cat. Num. AAA9606905

• Gene Names: BCL10; CLAP; mE10; CIPER; IMD37; c-E10; CARMEN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: BCL10; Polyclonal Antibody; BCL10 Antibody; AI132454; B cell CLL/lymphoma 10; B cell lymphoma/leukemia10; B-cell CLL/lymphoma 10; B-cell leukemia/lymphoma 10; B-cell lymphoma/leukemia 10; Bcl 10; Bcl-10; Bcl10; BCL10_HUMAN; c E10; c-E10; C81403; CARD containing apoptotic signaling protein; CARD containing molecule enhancing NF kappa B; CARD containing molecule enhancing NF kB; CARD containing molecule enhancing NF-kB; CARD containing molecule enhancing NFkB; CARD containing proapoptotic protein; CARD like apoptotic protein; CARD-containing apoptotic signaling protein; CARD-containing molecule enhancing NF-kappa-B; CARD-containing proapoptotic protein; CARD-like apoptotic protein; CARMEN; Caspase recruiting domain containing protein; caspase-recruiting domain-containing protein; cCARMEN; cE 10; cE10; CED 3/ICH 1 prodomain homologous E10 like regulator; CED-3/ICH-1 prodomain homologous E10-like regulator; CED3/ICH1 prodomain homologous E10 like regulator; Cellular E10; Cellular homolog of vCARMEN; Cellular-E10; CIPER; CLAP; hCLAP; Mammalian CARD containing adapter molecule E10; Mammalian CARD-containing adapter molecule E10; mE 10; mE10; R-RCD1; anti-BCL10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BCL10 antibody detects endogenous levels of total BCL10
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 233

• Applicable Applications for anti-BCL10 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human BCL10
• Subcellular Location: Cytoplasm > Perinuclear Region. Membrane Raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
• Tissue Specificity: Ubiquitous.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9606905 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of ovary using BCL10 antibody. The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-BCL10 antibody

Description: Bcl10/CIPER/CLAP/mE10 is a widely expressed CARD (caspase recruitment domain) containing protein shown to induce apoptosis and activate NF-kappaB (1-5). The CARD domain mediates self-oligomerization, interactions with other CARD proteins and is necessary for NF-kappaB activation, although the precise mechanism which Bcl10 regulates these processes is not fully understood. The discovery of Bcl10 came from observations of the chromosomal translocation t(1; 14)(p22; q32) from B cell lymphomas of the mucosa-associated lymphoid tissue (MALT) (1, 5). This translocation results in deregulated expression of a truncated form of Bcl10 which lacks apoptotic activity and enhances transformation. Studies from Bcl10 deficient mice demonstrate that Bcl10 is essential for the activation of NF-kappaB by T-and B-cell receptors (6). One third of Bcl10 deficient mice developed lethal exencephaly. Surviving mice were unaffected by various apoptotic stimuli, but were severely immunodeficient and defective in antigen receptor-induced NF-kappaBactiviation. PKC or T-cell receptor signaling results in a downregulation of Bcl10 protein levels, attenuating both NF-kappaB activation and cellular proliferation and also provides a negative feedback regulation of the NF-kappaB signaling to T cell signaling (7).
Function: Involved in adaptive immune response (PubMed:25365219). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1 (PubMed:18264101).
Subunit Structure: Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Self-associates by CARD-CARD interaction and forms a tight complex with MALT1. Interacts with other CARD-proteins such as CARD9, CARD10, CARD11 and CARD14. Forms a complex with CARD14 and MALT1; resulting in the formation of a CBM (CARD14-BCL10-MALT1) complex (PubMed:27113748). Binds caspase-9 with its C-terminal domain. Interacts with TRAF2 and BIRC2/c-IAP2. Interacts with PELI2 and SOCS3; these interactions may be mutually exclusive (By similarity). Interacts with CARD11 and MALT1; as part of a CBM (CARD11-BCL10-MALT1) complex involved in NF-kappa-B activation.
Post-translational Modifications: Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. Phosphorylated by IKBKB/IKKB.

NCBI and Uniprot Product Information

• NCBI GI #: 4502379
• NCBI GeneID: 8915
• NCBI Accession #: NP_003912.1
• NCBI GenBank Nucleotide #: NM_003921.5
• UniProt Accession #: O95999; Q5VUF1
• Molecular Weight: Observed: 26 kDa; Predicted: 27 kDa
• NCBI Official Full Name: B-cell lymphoma/leukemia 10 isoform 1
• NCBI Official Synonym Full Names: B cell CLL/lymphoma 10
• NCBI Official Symbol: BCL10
• NCBI Official Synonym Symbols: CLAP; mE10; CIPER; IMD37; c-E10; CARMEN
• NCBI Protein Information: B-cell lymphoma/leukemia 10
• UniProt Protein Name: B-cell lymphoma/leukemia 10
• Protein Family: B-cell lymphoma/leukemia
• UniProt Gene Name: BCL10
• UniProt Synonym Gene Names: CIPER; CLAP; Bcl-10; hCLAP; CIPER; cCARMEN; c-E10; mE10

NCBI Description

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Involved in adaptive immune response (PubMed:25365219). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1 (PubMed:18264101).