Immunofluorescence (IF)
(MBS9606183 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human ZAR1 Polyclonal Antibody | anti-ZAR1 antibody
ZAR1 Antibody
Gene Names
ZAR1; Z3CXXC6
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZAR1; Polyclonal Antibody; ZAR1 Antibody; Oocyte-specific maternal effect factor; zar1; ZAR1_HUMAN; zygote arrest 1; Zygote arrest protein 1; anti-ZAR1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ZAR1 antibody detects endogenous levels of total ZAR1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
424
Applicable Applications for anti-ZAR1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
EIA (peptide): 1:20000-1:40000
IF/ICC: 1:100-1:500
EIA (peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human ZAR1, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9606183 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9606183 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using ZAR1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using ZAR1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using ZAR1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using ZAR1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-ZAR1 antibody
Function: Essential for female fertility. May play a role in the oocyte-to-embryo transition (By similarity).
Similarity: Belongs to the ZAR1 family.
Similarity: Belongs to the ZAR1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46 KD
NCBI Official Full Name
zygote arrest protein 1
NCBI Official Synonym Full Names
zygote arrest 1
NCBI Official Symbol
ZAR1
NCBI Official Synonym Symbols
Z3CXXC6
NCBI Protein Information
zygote arrest protein 1
UniProt Protein Name
Zygote arrest protein 1
Protein Family
UniProt Gene Name
ZAR1
NCBI Description
This maternal effect gene is oocyte-specific and encodes a protein that is thought to function in the initiation of embryogenesis. A similar protein in mouse is required for female fertility. [provided by RefSeq, Jul 2013]
Uniprot Description
Essential for female fertility. May play a role in the oocyte-to-embryo transition ().
Research Articles on ZAR1
Similar Products
Product Notes
The ZAR1 zar1 (Catalog #AAA9606183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZAR1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZAR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500 EIA (peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the ZAR1 zar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZAR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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