Western Blot (WB)
(Western blot analysis of extracts from 293, using VANGL1 Antibody. Lane 1 was treated with the blocking peptide.)
Rabbit anti-Human, Mouse VANGL1 Polyclonal Antibody | anti-VANGL1 antibody
VANGL1 Antibody
Gene Names
VANGL1; LPP2; STB2; STBM2; KITENIN
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
VANGL1; Polyclonal Antibody; VANGL1 Antibody; ANGL planar cell polarity protein 1; KAI1 C-terminal interacting tetraspanin; KITENIN; Loop tail protein 2 homolog; Loop-tail protein 2 homolog; LPP2; MGC5338; STB2; STBM2; Strabismus 2; Strabismus; Drosophila; homolog of; 2; Van Gogh like protein 1; Van Gogh; 1; Van Gogh-like protein 1; Vang like 1 (van gogh; Drosophila); Vang like protein 1; Vang-like 1; Vang-like protein 1; VANG1_HUMAN; VANGL 1; Vangl1; anti-VANGL1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
VANGL1 antibody detects endogenous levels of total VANGL1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
522
Applicable Applications for anti-VANGL1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Membrane.
Tissue Specificity
According to PubMed:11956595, ubiquitously expressed. According to PubMed:12011995, expressed specifically in testis and ovary.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (85%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from 293, using VANGL1 Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using VANGL1 Antibody. Lane 1 was treated with the blocking peptide.)
Immunofluorescene (IF)
(MBS9606061 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9606061 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells, using VANGL1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells, using VANGL1 antibody.)
Related Product Information for anti-VANGL1 antibody
Subunit Structure: Heterodimer with VANGL2. Interacts through its C-terminal region with the N-terminal half of DVL1, DVL2 and DVL3. The PDZ domain of DVL1, DVL2 and DVL3 is required for the interaction (By similarity).
Similarity: Belongs to the Vang family.
Similarity: Belongs to the Vang family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 50 kD
Predicted: 60 kDa
Predicted: 60 kDa
NCBI Official Full Name
vang-like protein 1 isoform 2
NCBI Official Synonym Full Names
VANGL planar cell polarity protein 1
NCBI Official Symbol
VANGL1
NCBI Official Synonym Symbols
LPP2; STB2; STBM2; KITENIN
NCBI Protein Information
vang-like protein 1
UniProt Protein Name
Vang-like protein 1
Protein Family
UniProt Gene Name
VANGL1
UniProt Synonym Gene Names
STB2; LPP2
NCBI Description
This gene encodes a member of the tretraspanin family. The encoded protein may be involved in mediating intestinal trefoil factor induced wound healing in the intestinal mucosa. Mutations in this gene are associated with neural tube defects. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Research Articles on VANGL1
Similar Products
Product Notes
The VANGL1 vangl1 (Catalog #AAA9606061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VANGL1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VANGL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the VANGL1 vangl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VANGL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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