Immunofluorescence (IF)
(MBS9606047 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Mouse USP43 Polyclonal Antibody | anti-USP43 antibody
USP43 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
USP43; Polyclonal Antibody; USP43 Antibody; Deubiquitinating enzyme 43; Ubiquitin carboxyl-terminal hydrolase 43; ubiquitin specific peptidase 43; Ubiquitin specific protease 43; Ubiquitin thioesterase 43; Ubiquitin thiolesterase 43; Ubiquitin-specific-processing protease 43; UBP43; UBP43_HUMAN; Usp43; anti-USP43 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
USP43 antibody detects endogenous levels of total USP43
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1118
Applicable Applications for anti-USP43 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Tissue Specificity
Expressed in brain, aorta and lung at low levels.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (92%), Bovine (92%), Horse (100%), Sheep (82%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9606047 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9606047 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using USP43 Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using USP43 Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from JK cells, using USP43 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from JK cells, using USP43 antibody.)
Related Product Information for anti-USP43 antibody
Function: May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).
Similarity: Belongs to the peptidase C19 family.
Similarity: Belongs to the peptidase C19 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 125 kD
Predicted: 123 kDa
Predicted: 123 kDa
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 43 isoform 2
NCBI Official Synonym Full Names
ubiquitin specific peptidase 43
NCBI Official Symbol
USP43
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 43
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 43
Protein Family
UniProt Gene Name
USP43
Uniprot Description
May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins ().
Research Articles on USP43
Similar Products
Product Notes
The USP43 usp43 (Catalog #AAA9606047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP43 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's USP43 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the USP43 usp43 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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