Rabbit CXADR Polyclonal Antibody | anti-CXADR antibody

CXADR Antibody

Cat. Num. AAA9605429

• Gene Names: CXADR; CAR; HCAR; CAR4/6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CXADR; Polyclonal Antibody; CXADR Antibody; 46 kD coxsackievirus and adenovirus receptor CAR protein; 46 kD coxsackievirus and adenovirus receptor protein; CAR; CAR4/6; Coxsackie virus and adenovirus receptor; Coxsackie virus B receptor; Coxsackievirus and adenovirus receptor; Coxsackievirus B adenovirus receptor; Coxsackievirus B-adenovirus receptor; CVB3 binding protein; CVB3-binding protein; Cxadr; CXAR_HUMAN; hCAR; HCVADR; anti-CXADR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CXADR antibody detects endogenous levels of total CXADR
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 252

• Applicable Applications for anti-CXADR antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Secreted and Cell Membrane. Cell Junction > Tight Junction. Cell Junction > Adherens Junction. Basolateral Cell Membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
• Tissue Specificity: Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
• Predicted Cross Reactivity: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
• Similarity: Pig (100%), Bovine (90%), Sheep (90%), Rabbit (90%), Dog (100%), Chicken (83%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from COLO cells using CXADR antibody.)

Immunofluorescene (IF)

(MBS9605429 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-CXADR antibody

Function: Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.
Subunit Structure: Monomer. May form homodimer. Interacts with LNX, BAIAP1, DLG4, PRKCABP, TJP1 and CTNNB1. Interacts with MPDZ; recruits MPDZ to intercellular contact sites. Interacts with JAML (homodimeric form). Secreted isoform 3, isoform 4 and isoform 5 can interact with the extracellular domain of the receptor.
Post-translational Modifications: N-glycosylated. Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
Similarity: The Ig-like C2-type 1 domain mediates homodimerization and interaction with JAML.The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.

NCBI and Uniprot Product Information

• NCBI GI #: 333470703
• NCBI GeneID: 1525
• NCBI Accession #: NP_001193992.1
• NCBI GenBank Nucleotide #: NM_001207063.1
• UniProt Accession #: P78310; O00694; Q8WWT6; Q8WWT7; Q8WWT8; Q9UKV4; B2R8V8; B7WPI3; D3YHP0
• Molecular Weight: Observed: 40 kD; Predicted: 41 kDa
• NCBI Official Full Name: coxsackievirus and adenovirus receptor isoform 2
• NCBI Official Synonym Full Names: CXADR, Ig-like cell adhesion molecule
• NCBI Official Symbol: CXADR
• NCBI Official Synonym Symbols: CAR; HCAR; CAR4/6
• NCBI Protein Information: coxsackievirus and adenovirus receptor
• UniProt Protein Name: Coxsackievirus and adenovirus receptor
• Protein Family: CXADR-like membrane protein
• UniProt Gene Name: CXADR
• UniProt Synonym Gene Names: CAR; CAR; hCAR

NCBI Description

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

Uniprot Description

Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.