Immunofluorescence (IF)
(MBS9605394 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit CSPG5 Polyclonal Antibody | anti-CSPG5 antibody
CSPG5 Antibody
Gene Names
CSPG5; NGC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CSPG5; Polyclonal Antibody; CSPG5 Antibody; Acidic leucine rich EGF like domain containing brain protein; Acidic leucine-rich EGF-like domain-containing brain protein; Caleb; Chondroitin sulfate proteoglycan 5 (neuroglycan C); Chondroitin sulfate proteoglycan 5; CSPG5_HUMAN; MGC44034; Neuroglycan C; Ngc; OTTHUMP00000164796; OTTHUMP00000164797; OTTHUMP00000210429; anti-CSPG5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CSPG5 antibody detects endogenous levels of total CSPG5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
566
Applicable Applications for anti-CSPG5 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane. Endoplasmic Reticulum Membrane. Golgi Apparatus Membrane. In neurons, localizes to synaptic junctions (By similarity). Also detected in the endoplasmic reticulum and the golgi (By similarity). Partially enriched in lipid rafts.
Tissue Specificity
Restricted to brain (at protein level).
Predicted Cross Reactivity
Rabbit, Dog
Similarity
Rabbit (82%), Dog (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605394 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9605394 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using CSPG5 antibody., The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using CSPG5 antibody., The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-CSPG5 antibody
Function: May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.
Subunit Structure: Binds TNR and probably TNC (By similarity). Interacts with ERBB3 and GOPC.
Post-translational Modifications: N-glycosylated. O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation (By similarity). Phosphorylated; in intracellular and extracellular parts.
Subunit Structure: Binds TNR and probably TNC (By similarity). Interacts with ERBB3 and GOPC.
Post-translational Modifications: N-glycosylated. O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation (By similarity). Phosphorylated; in intracellular and extracellular parts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 60 kD
Predicted: 61 kDa
Predicted: 61 kDa
NCBI Official Full Name
chondroitin sulfate proteoglycan 5 isoform 3
NCBI Official Synonym Full Names
chondroitin sulfate proteoglycan 5
NCBI Official Symbol
CSPG5
NCBI Official Synonym Symbols
NGC
NCBI Protein Information
chondroitin sulfate proteoglycan 5
UniProt Protein Name
Chondroitin sulfate proteoglycan 5
Protein Family
UniProt Gene Name
CSPG5
UniProt Synonym Gene Names
CALEB; NGC
NCBI Description
The protein encoded by this gene is a proteoglycan that may function as a neural growth and differentiation factor. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
Uniprot Description
May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.
Research Articles on CSPG5
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Product Notes
The CSPG5 cspg5 (Catalog #AAA9605394) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CSPG5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CSPG5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CSPG5 cspg5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSPG5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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