Immunohistochemistry (IHC)
(MBS9604334 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human MRGRD Polyclonal Antibody | anti-MRGRD antibody
MRGRD Antibody
Gene Names
MRGPRD; MRGD; TGR7
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
MRGRD; Polyclonal Antibody; MRGRD Antibody; Beta alanine receptor; Beta-alanine receptor; G protein coupled receptor TGR7; G-protein coupled receptor TGR7; mas related G protein coupled MRGD; Mas related G protein coupled receptor member D; MAS related GPR member D; Mas-related G-protein coupled receptor member D; MRGD; Mrgprd; MRGRD_HUMAN; TGR7; anti-MRGRD antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
MRGRD antibody detects endogenous levels of total MRGRD
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
321
Applicable Applications for anti-MRGRD antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA: 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight.
IHC: 1:50-1:200
ELISA: 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight.
Immunogen
A synthesized peptide derived from human MRGRD, corresponding to a region within C-terminal amino acids.
Immunohistochemistry (IHC)
(MBS9604334 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604334 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of MRGRD expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of MRGRD expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-MRGRD antibody
Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41 kDa;;10% SDS-PAGE
NCBI Official Full Name
mas-related G-protein coupled receptor member D
NCBI Official Synonym Full Names
MAS related GPR family member D
NCBI Official Symbol
MRGPRD
NCBI Official Synonym Symbols
MRGD; TGR7
NCBI Protein Information
mas-related G-protein coupled receptor member D
UniProt Protein Name
Mas-related G-protein coupled receptor member D
UniProt Gene Name
MRGPRD
UniProt Synonym Gene Names
MRGD
Uniprot Description
May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca2+ influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i).
Research Articles on MRGRD
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Product Notes
The MRGRD mrgprd (Catalog #AAA9604334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRGRD Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MRGRD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA: 1:20000-1:40000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight. Researchers should empirically determine the suitability of the MRGRD mrgprd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRGRD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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