Immunohistochemistry (IHC)
(MBS9604021 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse HSPB9 Polyclonal Antibody | anti-HSPB9 antibody
HSPB9 Antibody
Gene Names
HSPB9; CT51
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HSPB9; Polyclonal Antibody; HSPB9 Antibody; Cancer/testis antigen 51; CT51; Heat shock protein alpha crystallin related B9; Heat shock protein beta 9; Heat shock protein beta-9; HspB9; HSPB9_HUMAN; Small heat shock protein B9; anti-HSPB9 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
HSPB9 antibody detects endogenous levels of total HSPB9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
159
Applicable Applications for anti-HSPB9 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
Tissue Specificity
Testis specific.
Predicted Cross Reactivity
Pig, Horse, Rabbit, Dog
Similarity
Pig (80%), Horse (80%), Rabbit (88%), Dog (88%)
Conjugation
Unconjugated
Immunohistochemistry (IHC)
(MBS9604021 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604021 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of HSPB9 expression in A549 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of HSPB9 expression in A549 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-HSPB9 antibody
Similarity: Belongs to the small heat shock protein (HSP20) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 17 kDa
Predicted: 18 kDa
Predicted: 18 kDa
NCBI Official Full Name
heat shock protein beta-9
NCBI Official Synonym Full Names
heat shock protein family B (small) member 9
NCBI Official Symbol
HSPB9
NCBI Official Synonym Symbols
CT51
NCBI Protein Information
heat shock protein beta-9
UniProt Protein Name
Heat shock protein beta-9
Protein Family
UniProt Gene Name
HSPB9
UniProt Synonym Gene Names
HspB9; CT51
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Product Notes
The HSPB9 hspb9 (Catalog #AAA9604021) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPB9 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HSPB9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HSPB9 hspb9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPB9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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