Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using MRFAP1 antibody.)
Rabbit anti-Human, Mouse MRFAP1 Polyclonal Antibody | anti-MRFAP1 antibody
MRFAP1 Antibody
Gene Names
MRFAP1; PGR1; PAM14; PGR1`
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MRFAP1; Polyclonal Antibody; MRFAP1 Antibody; Mof4 family associated protein 1; MOFA1_HUMAN; MORF4 family associated protein 1; MORF4 family-associated protein 1; Mrfap1; OTTHUMP00000155159; OTTHUMP00000217763; PAM14; PGR1; Protein associated with MRG 14 kDa; Protein associated with MRG of 14 kDa; Protein PGR1; T cell activation protein; anti-MRFAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
MRFAP1 antibody detects endogenous levels of MRFAP1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
127
Applicable Applications for anti-MRFAP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human MRFAP1.
Subcellular Location
Nucleus. Cytoplasm, Perinuclear Region. Colocalizes with MORF4L1 to cell nuclei.
Predicted Cross Reactivity
Bovine, Dog
Similarity
Bovine (83%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using MRFAP1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using MRFAP1 antibody.)
Immunohistochemistry (IHC)
(MBS9603652 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603652 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-MRFAP1 antibody
Subunit Structure: Found in a complex composed of MORF4L1, MRFAP1 and RB1. Interacts via its N-terminus with MORF4L1. Interacts with CSTB and MORF4L2.
Similarity: Belongs to the MORF4 family-associated protein family.
Similarity: Belongs to the MORF4 family-associated protein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 15 kDa
Predicted: 15 kDa
Predicted: 15 kDa
NCBI Official Full Name
MORF4 family-associated protein 1 isoform a
NCBI Official Synonym Full Names
Morf4 family associated protein 1
NCBI Official Symbol
MRFAP1
NCBI Official Synonym Symbols
PGR1; PAM14; PGR1`
NCBI Protein Information
MORF4 family-associated protein 1
UniProt Protein Name
MORF4 family-associated protein 1
Protein Family
UniProt Gene Name
MRFAP1
NCBI Description
This gene encodes an intracellular protein that interacts with members of the MORF4/MRG (mortality factor on chromosome 4/MORF4 related gene) family and the tumor suppressor Rb (retinoblastoma protein.) The protein may play a role in senescence, cell growth and immortalization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
Research Articles on MRFAP1
Similar Products
Product Notes
The MRFAP1 mrfap1 (Catalog #AAA9603652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRFAP1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MRFAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the MRFAP1 mrfap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRFAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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