Rabbit CIN85 Polyclonal Antibody | anti-CIN85 antibody

CIN85 Antibody

Cat. Num. AAA9603593

• Gene Names: SH3KBP1; HSB1; CIN85; GIG10; HSB-1; MIG18; CD2BP3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CIN85; Polyclonal Antibody; CIN85 Antibody; c Cbl interacting protein; Cbl interacting protein; Cbl interacting protein of 85 kDa; Cbl-interacting protein of 85 kDa; CD2 binding protein 3; CD2-binding protein 3; CD2BP3; CIN 85; GIG 10; GIG10; HSB 1; HSB-1; HSB1; Human Src family kinase binding protein 1; Human Src family kinase-binding protein 1; MIG 18; MIG18; Migration inducing gene 18; Migration inducing gene 18 protein; SH3 domain kinase binding protein 1; SH3 domain-containing kinase-binding protein 1; SH3BP 1; SH3K1_HUMAN; SH3KBP1; Src family kinase binding protein 1; src related kinase binding protein 1; anti-CIN85 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CIN85 antibody detects endogenous levels of CIN85.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 628

• Applicable Applications for anti-CIN85 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human CIN85.
• Subcellular Location: Cytoskeleton
• Tissue Specificity: Ubiquitously expressed. Also expressed in some cancer cell lines.
• Predicted Cross Reactivity: Pig, Zebrafish, Rabbit, Dog, Chicken
• Similarity: Pig (100%), Zebrafish (90%), Rabbit (100%), Dog (100%), Chicken (90%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603593 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of fetal human brain tissue, using CIN85 antibody.)

Related Product Information for anti-CIN85 antibody

Function: Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration.
Subunit Structure: Can self-associate and form homotetramers. Interacts with CD2, F-actin capping protein, PIK3R3, GRB2, EGFR, MET, BLNK, MAP3K4, PDCD6IP, SPRY2, ARHGAP17, ARHGAP27, MAGI2, CRK, BCAR1, SOS1, ASAP1, ARAP3, HIP1R, SYNJ2, INPP5D and STAP1. Interacts with CBL and CBLB, but does not interact with CBLC. Two molecules of SH3KBP1 seem to bind through their respective SH3 1 domain to one molecule of CBLB. The interaction with CBL or CBLB and EGFR is increased upon EGF stimulation. The interaction with CBL is attenuated by PDCD6IP. Interacts through its proline-rich region with the SH3 domain of endophilins SH3GL1, SH3GL2 and SH3GL3. The SH3KBP1-endophilin complex seems to associate with a complex containing the phosphorylated receptor (EGFR or MET) and phosphorylated CBL. Probably associates with ASAP1 and phosphorylated EGFR. Probably part of a complex consisting of at least SH3KBP1, ASAP1 and ARAP3. Interacts with focal adhesion kinases PTK2/FAK1 AND PTK2B/PYK2, probably as a dimer. Interacts with DAB2 and probably associates with chathrin through its interaction with DAB2. Part of a complex consisting of SH3KBP1, DAB2, and clathrin heavy chain. DAB2 and clathrin dissociate from SH3KBP1 following growth factor treatment, enabling interaction with CBL. Interacts with DDN and probably associates with MAGI2 through its interaction with DDN. Interacts with the SH3 domains of SRC tyrosine-protein kinases SRC, LCK, LYN, FGR, FYN and HCK. Interacts with TRADD, BIRC2, TRAF1, TRAF2 and TNFR1, and the association with a TNFR1-associated complex upon stimulation with TNF-alpha seems to be mediated by SRC. Interacts (via SH3 domains) with SHKBP1 (via PXXXPR motifs) (By similarity). Interaction with CBL is abolished in the presence of SHKBP1 (By similarity). Interacts (via SH3 domains) with ZFP36 (via extreme C-terminal region) (PubMed:20221403). Interacts with MAP3K4; this interaction enhances the association with ZFP36 (PubMed:20221403).
Post-translational Modifications: Monoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 67514036
• NCBI GeneID: 30011
• NCBI Accession #: NP_001019837.1
• NCBI GenBank Nucleotide #: NM_001024666.2
• UniProt Accession #: Q96B97; Q5JPT4; Q5JPT5; Q8IWX6; Q8IX98; Q96RN4; Q9NYR0; B7Z1D5
• Molecular Weight: Observed: 85 kDa; Predicted: 74 kDa
• NCBI Official Full Name: SH3 domain-containing kinase-binding protein 1 isoform b
• NCBI Official Synonym Full Names: SH3 domain containing kinase binding protein 1
• NCBI Official Symbol: SH3KBP1
• NCBI Official Synonym Symbols: HSB1; CIN85; GIG10; HSB-1; MIG18; CD2BP3
• NCBI Protein Information: SH3 domain-containing kinase-binding protein 1
• UniProt Protein Name: SH3 domain-containing kinase-binding protein 1
• UniProt Gene Name: SH3KBP1
• UniProt Synonym Gene Names: CIN85; CD2BP3; HSB-1

NCBI Description

This gene encodes an adapter protein that contains one or more N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]

Uniprot Description

Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration.