Western Blot (WB)
(Western blot analysis of extracts from 3T3, using ARL8B antibody. Lane 1 was treated with the blocking peptide.)
Rabbit anti-Human, Mouse ARL8B Polyclonal Antibody | anti-ARL8B antibody
ARL8B Antibody
Gene Names
ARL8B; Gie1; ARL10C
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ARL8B; Polyclonal Antibody; ARL8B Antibody; ADP ribosylation factor like 10C; ADP-ribosylation factor-like protein 10C; ADP-ribosylation factor-like protein 8B; ARL10C; ARL8B_HUMAN; FLJ10702; Gie1; Novel small G protein indispensable for equal chromosome segregation 1; anti-ARL8B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ARL8B antibody detects endogenous levels of ARL8B.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
186
Applicable Applications for anti-ARL8B antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human ARL8B.
Subcellular Location
Late Endosome Membrane. Lysosome Membrane. Cytoplasm > Cytoskeleton > Spindle. According to PubMed:15331635, it localizes with microtubules at the spindle mid-zone during mitosis.
Tissue Specificity
Ubiquitously expressed.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from 3T3, using ARL8B antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 3T3, using ARL8B antibody. Lane 1 was treated with the blocking peptide.)
Immunohistochemistry (IHC)
(MBS9603578 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603578 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of mouse brain tissue, using ARL8B antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse brain tissue, using ARL8B antibody.)
Related Product Information for anti-ARL8B antibody
Function: May play a role in lysosome motility (PubMed:16537643, PubMed:25898167). May play a role in chromosome segregation (PubMed:15331635).
Subunit Structure: Interacts with tubulin (PubMed:15331635, Ref. 16). Interacts with BORCS5; recruits ARL8B to lysosomes (PubMed:25898167).
Similarity: Belongs to the small GTPase superfamily. Arf family.
Subunit Structure: Interacts with tubulin (PubMed:15331635, Ref. 16). Interacts with BORCS5; recruits ARL8B to lysosomes (PubMed:25898167).
Similarity: Belongs to the small GTPase superfamily. Arf family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 22 kDa
Predicted: 22 kDa
Predicted: 22 kDa
NCBI Official Full Name
ADP-ribosylation factor-like protein 8B
NCBI Official Synonym Full Names
ADP ribosylation factor like GTPase 8B
NCBI Official Symbol
ARL8B
NCBI Official Synonym Symbols
Gie1; ARL10C
NCBI Protein Information
ADP-ribosylation factor-like protein 8B
UniProt Protein Name
ADP-ribosylation factor-like protein 8B
Protein Family
UniProt Gene Name
ARL8B
UniProt Synonym Gene Names
ARL10C; GIE1
Uniprot Description
May play a role in lysosome motility (PubMed:16537643, PubMed:25898167). May play a role in chromosome segregation (PubMed:15331635).
Research Articles on ARL8B
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Product Notes
The ARL8B arl8b (Catalog #AAA9603578) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARL8B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ARL8B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ARL8B arl8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARL8B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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