Immunohistochemistry (IHC)
(MBS9603015 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human CD300LB Polyclonal Antibody | anti-CD300LB antibody
CD300LB Antibody
Gene Names
CD300LB; CLM7; CLM-7; IREM3; TREM5; CD300b; IREM-3; TREM-5; CMRF35-A2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CD300LB; Polyclonal Antibody; CD300LB Antibody; CD_antigen=CD300b; CD300 antigen-like family member B; CD300 molecule-like family member b; CD300B; CLM 7; CLM7; CLM7_HUMAN; CMRF35 A2; CMRF35-like molecule 7; CMRF35A2; Immune receptor expressed on myeloid cells 3; IREM 3; IREM3; Leukocyte mono-Ig-like receptor 5; LMIR5; TREM 5; TREM5; Triggering receptor expressed on myeloid cells 5; UNQ2530/PRO6029; anti-CD300LB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CD300LB antibody detects endogenous levels of total CD300LB
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
201
Applicable Applications for anti-CD300LB antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human CD300LB
Subcellular Location
Cell Membrane
Tissue Specificity
Expressed exclusively in myeloid lineages.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603015 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603015 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of CD300LB using HT-29 whole cell lysates)
Western Blot (WB)
(Western blot analysis of CD300LB using HT-29 whole cell lysates)
Related Product Information for anti-CD300LB antibody
Function: Acts as an activating immune receptor through its interaction with ITAM-bearing adapter TYROBP, and also independently by recruitment of GRB2.
Subunit Structure: Interacts with TYROBP, which enhances cell surface expression and activation properties. Interacts with GRB2 in the presence of FYN.
Post-translational Modifications: Phosphorylation on Tyr-188 by FYN is required for interaction with GRB2.
Similarity: Belongs to the CD300 family.
Subunit Structure: Interacts with TYROBP, which enhances cell surface expression and activation properties. Interacts with GRB2 in the presence of FYN.
Post-translational Modifications: Phosphorylation on Tyr-188 by FYN is required for interaction with GRB2.
Similarity: Belongs to the CD300 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 23 kDa
Predicted: 23 kDa
Predicted: 23 kDa
NCBI Official Full Name
CMRF35-like molecule 7
NCBI Official Synonym Full Names
CD300 molecule like family member b
NCBI Official Symbol
CD300LB
NCBI Official Synonym Symbols
CLM7; CLM-7; IREM3; TREM5; CD300b; IREM-3; TREM-5; CMRF35-A2
NCBI Protein Information
CMRF35-like molecule 7
UniProt Protein Name
CMRF35-like molecule 7
Protein Family
UniProt Gene Name
CD300LB
UniProt Synonym Gene Names
CD300B; CLM7; CMRF35A2; IREM3; LMIR5; TREM5; CLM-7; IREM-3; TREM-5
NCBI Description
CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells (Martinez-Barriocanal and Sayos, 2006 [PubMed 16920917]).[supplied by OMIM, Mar 2008]
Uniprot Description
Acts as an activating immune receptor through its interaction with ITAM-bearing adapter TYROBP, and also independently by recruitment of GRB2.
Research Articles on CD300LB
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Product Notes
The CD300LB cd300lb (Catalog #AAA9603015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD300LB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD300LB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the CD300LB cd300lb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD300LB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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