Mouse anti-Human TUBB3 Monoclonal Antibody | anti-TUBB3 antibody

TUBB3 Antibody

Cat. Num. AAA9602733

• Gene Names: TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA, Flow Cytometry, Functional Assay
• Purity: Affinity-Chromatography
• Synonyms: TUBB3; Monoclonal Antibody; TUBB3 Antibody; beta 3 tubulin; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; QccE-11995; QccE-15186; TBB3_HUMAN; Tubb 3; TUBB4; Tubulin beta 3; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; anti-TUBB3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: TUBB3 antibody detects endogenous levels of total TUBB3
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 378

• Applicable Applications for anti-TUBB3 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: ELISA: 1:10000; WB: 1:500-1:2000; ICC: 1:200-1:1000; FC/FACS: 1:200-1:400
• Immunogen: Purified recombinant fragment of human TUBB3 expressed in E. Coli
• Subcellular Location: Cytoplasm > Cytoskeleton.
• Tissue Specificity: Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Figure 1: Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.)

Related Product Information for anti-TUBB3 antibody

Description: Tubulin, beta 3, also known as TUBB3. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, alpha-and beta-tubulin, are usually products of separate genes. The beta-tubulin family includes six expressed genes that produce the polypeptide isoforms known as Classes I through VI, each of which have a distinct pattern of expression. Class III beta-tubulin is found in neurons and mammalian testis cells and is widely used as a neuronal marker in developmental neurobiology, neoplasia, and stem cell research. Class III beta-tubulin expression in neuronal and neuroblastic tumors is differentiation dependent, and its expression in certain non-neuronal neoplasms has been associated with poor prognosis and/or resistance to chemotherapy.
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Subunit Structure: Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Post-translational Modifications: Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Similarity: The highly acidic C-terminal region may bind cations such as calcium. Belongs to the tubulin family.

NCBI and Uniprot Product Information

• NCBI GI #: 308235963
• NCBI GeneID: 10381
• NCBI Accession #: NP_001184110.1
• NCBI GenBank Nucleotide #: NM_001197181.1
• UniProt Accession #: Q13509; Q9BTZ0; Q9BW10; A8K854
• Molecular Weight: Observed: 50 kDa; Predicted: 51 kDa
• NCBI Official Full Name: tubulin beta-3 chain isoform 2
• NCBI Official Synonym Full Names: tubulin beta 3 class III
• NCBI Official Symbol: TUBB3
• NCBI Official Synonym Symbols: CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
• NCBI Protein Information: tubulin beta-3 chain
• UniProt Protein Name: Tubulin beta-3 chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB3
• UniProt Synonym Gene Names: TUBB4

NCBI Description

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.