Western Blot (WB)
(Figure 1: Western blot analysis using LMO2 mAb against human LMO2 recombinant protein. (Expected MW is 43.9 kDa))
Mouse anti-Human LMO2 Monoclonal Antibody | anti-LMO2 antibody
LMO2 Antibody
Gene Names
LMO2; TTG2; LMO-2; RBTN2; RHOM2; RBTNL1
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Affinity-Chromatography
Synonyms
LMO2; Monoclonal Antibody; LMO2 Antibody; Cysteine rich protein TTG 2; Cysteine rich protein TTG2; Cysteine-rich protein TTG-2; LIM domain only 2 (rhombotin like 1); LIM domain only 2; LIM domain only protein 2; LMO 2; LMO-2; lmo2; RBTN 2; RBTN L1; RBTN2; RBTN2_HUMAN; RBTNL 1; RBTNL1; RHOM 2; RHOM2; Rhombotin 2; Rhombotin like 1; Rhombotin-2; Rhombotin2; T cell translocation gene 2; T cell translocation protein 2; T-cell translocation protein 2; TTG 2; TTG2; anti-LMO2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
LMO2 antibody detects endogenous levels of total LMO2
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
158
Applicable Applications for anti-LMO2 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
Immunogen
Purified recombinant fragment of human LMO2 expressed in E. Coli
Subcellular Location
Nucleus.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using LMO2 mAb against human LMO2 recombinant protein. (Expected MW is 43.9 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using LMO2 mAb against human LMO2 recombinant protein. (Expected MW is 43.9 kDa))
Related Product Information for anti-LMO2 antibody
Description: LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved.
Function: Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
Subunit Structure: Interacts via its LIM domains with ELF2 and LDB1. Also interacts with basic helix-loop-helix protein TAL1/SCL and can assemble in a complex with LMO2 and TAL1/SCL (By similarity). Interacts with BEX2 and KDM5A.
Similarity: The second LIM zinc-binding domain interacts with KDM5A.
Function: Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
Subunit Structure: Interacts via its LIM domains with ELF2 and LDB1. Also interacts with basic helix-loop-helix protein TAL1/SCL and can assemble in a complex with LMO2 and TAL1/SCL (By similarity). Interacts with BEX2 and KDM5A.
Similarity: The second LIM zinc-binding domain interacts with KDM5A.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 18.4 kDa
Predicted: 19 kDa
Predicted: 19 kDa
NCBI Official Full Name
rhombotin-2 isoform 2
NCBI Official Synonym Full Names
LIM domain only 2
NCBI Official Symbol
LMO2
NCBI Official Synonym Symbols
TTG2; LMO-2; RBTN2; RHOM2; RBTNL1
NCBI Protein Information
rhombotin-2
UniProt Protein Name
Rhombotin-2
Protein Family
UniProt Gene Name
LMO2
UniProt Synonym Gene Names
RBTN2; RBTNL1; RHOM2; TTG2; LMO-2
NCBI Description
LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Nov 2008]
Uniprot Description
Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
Research Articles on LMO2
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Product Notes
The LMO2 lmo2 (Catalog #AAA9602305) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LMO2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LMO2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:10000 WB: 1:500-1:2000 FC/FACS: 1:200-1:400. Researchers should empirically determine the suitability of the LMO2 lmo2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LMO2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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