Western Blot (WB)
(Western blot analysis of TRalpha using SKOV3 whole cell lysates)
Rabbit THR alpha Polyclonal Antibody | anti-THRA antibody
THR alpha Antibody
Gene Names
THRA; AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
THR alpha; Polyclonal Antibody; THR alpha Antibody; AR7; c-erbA-1; C-erbA-alpha; CHNG6; EAR-7; EAR7; ERB-T-1; ERBA; ERBA-related 7; ERBA1; NR1A1; Nuclear receptor subfamily 1 group A member 1; THA_HUMAN; THRA; THRA1; THRA2; Thyroid hormone receptor alpha; thyroid hormone receptor; alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog; avian); thyroid normone nuclear receptor alpha variant 1; triiodothyronine receptor; V-erbA-related protein 7; anti-THRA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TRalpha antibody detects endogenous levels of total TRalpha
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
451
Applicable Applications for anti-THRA antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA :1:20000-1:40000
The optimal dilutions should be determined by the end user.
Important Note: For western blots , incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night.
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA :1:20000-1:40000
The optimal dilutions should be determined by the end user.
Important Note: For western blots , incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night.
Immunogen
A synthesized peptide derived from human TRalpha, corresponding to a region within N-terminal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of TRalpha using SKOV3 whole cell lysates)
Western Blot (WB)
(Western blot analysis of TRalpha using SKOV3 whole cell lysates)
Immunofluorescene (IF)
(MBS9602261 staining SKOV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9602261 staining SKOV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-THRA antibody
Function: Isoform Alpha-1: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Subunit Structure: Binds DNA as a dimer; homodimer and heterodimer with RXRB. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Probably interacts with SFPQ. Interacts with C1D. Interacts with AKAP13. Interacts with TP53INP2. Interacts with PER2.
Similarity: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Belongs to the nuclear hormone receptor family. NR1 subfamily.
Subunit Structure: Binds DNA as a dimer; homodimer and heterodimer with RXRB. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Probably interacts with SFPQ. Interacts with C1D. Interacts with AKAP13. Interacts with TP53INP2. Interacts with PER2.
Similarity: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Belongs to the nuclear hormone receptor family. NR1 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55 kDa
NCBI Official Full Name
thyroid hormone receptor alpha isoform 3
NCBI Official Synonym Full Names
thyroid hormone receptor alpha
NCBI Official Symbol
THRA
NCBI Official Synonym Symbols
AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
NCBI Protein Information
thyroid hormone receptor alpha
UniProt Protein Name
Thyroid hormone receptor alpha
Protein Family
UniProt Gene Name
THRA
UniProt Synonym Gene Names
EAR7; ERBA1; NR1A1; THRA1; THRA2; EAR-7
NCBI Description
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
Isoform Alpha-1: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Research Articles on THRA
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Product Notes
The THRA thra (Catalog #AAA9602261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The THR alpha Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's THR alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF: 1:100-1:500 ICC: 1:100-1:500 ELISA :1:20000-1:40000The optimal dilutions should be determined by the end user. Important Note: For western blots, incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night. Researchers should empirically determine the suitability of the THRA thra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "THR alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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