Western Blot (WB)
(Western blot analysis NFATc2IP using K562 whole cell lysates)
Rabbit anti-Human NFATc2IP Polyclonal Antibody | anti-NFATc2IP antibody
NFATc2IP Antibody
Gene Names
NFATC2IP; ESC2; NIP45; RAD60
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NFATc2IP; Polyclonal Antibody; NFATc2IP Antibody; 45 kDa NF-AT-interacting protein; 45 kDa NFAT-interacting protein; cytoplasmic 2-interacting protein; ESC2; NF2IP_HUMAN; NFAT-interacting protein; 45-KD; NFATC2-interacting protein; Nfatc2ip; NIP45; Nuclear factor of activated T-cells; nuclear factor of activated T-cells; cytoplasmic; calcineurin-dependent 2 interacting protein; RAD60; anti-NFATc2IP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
NFATc2IP antibody detects endogenous levels of total NFATc2IP
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
419
Applicable Applications for anti-NFATc2IP antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human NFATc2IP
Subcellular Location
Nucleus
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis NFATc2IP using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis NFATc2IP using K562 whole cell lysates)
Immunofluorescene (IF)
(MBS9602171 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9602171 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-NFATc2IP antibody
Function: In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression (By similarity). Down-regulates formation of poly-SUMO chains by UBE2I/UBC9 (By similarity).
Subunit Structure: Interacts with NFATC2, TRAF1, TRAF2 and PRMT1. Interacts with UBE2I/UBC9 (By similarity).
Post-translational Modifications: Methylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production.
Subunit Structure: Interacts with NFATC2, TRAF1, TRAF2 and PRMT1. Interacts with UBE2I/UBC9 (By similarity).
Post-translational Modifications: Methylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 48 kDa
Predicted: 46 kDa
Predicted: 46 kDa
NCBI Official Full Name
NFATC2-interacting protein
NCBI Official Synonym Full Names
nuclear factor of activated T cells 2 interacting protein
NCBI Official Symbol
NFATC2IP
NCBI Official Synonym Symbols
ESC2; NIP45; RAD60
NCBI Protein Information
NFATC2-interacting protein
UniProt Protein Name
NFATC2-interacting protein
Protein Family
UniProt Gene Name
NFATC2IP
UniProt Synonym Gene Names
NIP45; 45 kDa NFAT-interacting protein
Uniprot Description
In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression (). Down-regulates formation of poly-SUMO chains by UBE2I/UBC9 ().
Research Articles on NFATc2IP
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Product Notes
The NFATc2IP nfatc2ip (Catalog #AAA9602171) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFATc2IP Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFATc2IP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the NFATc2IP nfatc2ip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFATc2IP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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