ELISA (EIA)
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody)
Rabbit Ephrin B1/B2/B3 Polyclonal Antibody | anti-EFNB1/EFNB2/EFNB3 antibody
Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody
Gene Names
EFNB1; CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
Ephrin B1/B2/B3; Polyclonal Antibody; Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody; CEK5 ligand; CEK5 receptor ligand; CEK5-L; CEL5-L; EFL-3; EFNB1; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; EPL2; EPLG2; Ephrin-B1 precursor; LERK-2; LERK2; STRA1; STRA1 protein; kinase ephrin-B1; anti-EFNB1/EFNB2/EFNB3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody detects endogenous levels of EFNB1/EFNB2/EFNB3 only when phosphorylated at Tyr324.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
346
Applicable Applications for anti-EFNB1/EFNB2/EFNB3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
IHC: 1:50~1:100
ELISA: 1:10000
Target Modification
Phospho
Modification Sites
H:Y324/311/318; M:Y323/314/318; R:Y323/-/318
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
ELISA (EIA)
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody)
ELISA (EIA)
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody)
Western Blot (WB)
(Western blot analysis of lysates from K562 cells treated with serum 20% 15', using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody.)
Western Blot (WB)
(Western blot analysis of lysates from K562 cells treated with serum 20% 15', using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38 kDa
NCBI Official Full Name
ephrin-B1
NCBI Official Synonym Full Names
ephrin B1
NCBI Official Symbol
EFNB1
NCBI Official Synonym Symbols
CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2
NCBI Protein Information
ephrin-B1
UniProt Protein Name
Ephrin-B1
UniProt Gene Name
EFNB1
UniProt Synonym Gene Names
EFL3; EPLG2; LERK2; ELK-L; LERK-2
NCBI Description
The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008]
Uniprot Description
Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons ().
Research Articles on EFNB1/EFNB2/EFNB3
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Product Notes
The EFNB1/EFNB2/EFNB3 efnb1 (Catalog #AAA9508593) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ephrin B1/B2/B3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000. Researchers should empirically determine the suitability of the EFNB1/EFNB2/EFNB3 efnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ephrin B1/B2/B3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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