Rabbit Histone H4 Polyclonal Antibody | anti-HIST1H4A antibody

Histone H4 (Acetyl-Lys12) Antibody

Cat. Num. AAA9507793

• Gene Names: HIST2H4B; H4/o
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
• Synonyms: Histone H4; Polyclonal Antibody; Histone H4 (Acetyl-Lys12) Antibody; H4F2; HIST; HIST1H4A; anti-HIST1H4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Histone H4 (Acetyl-Lys12) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at Lys12.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 103

• Applicable Applications for anti-HIST1H4A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000
• Target Modification: Acetyl
• Modification Sites: H:K12; M:K12; R:K12
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12.
• Immunogen Range: Oct-59
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys12) Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 77539758
• NCBI GeneID: 554313
• NCBI Accession #: NP_001029249.1
• NCBI GenBank Nucleotide #: NM_001034077.4
• UniProt Accession #: P62805; P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0
• Molecular Weight: 11 kDa
• NCBI Official Full Name: histone H4
• NCBI Official Synonym Full Names: histone cluster 2 H4 family member b
• NCBI Official Symbol: HIST2H4B
• NCBI Official Synonym Symbols: H4/o
• NCBI Protein Information: histone H4
• UniProt Protein Name: Histone H4
• Protein Family: Histone
• UniProt Gene Name: HIST1H4A
• UniProt Synonym Gene Names: H4/A; H4FA

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Aug 2015]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.