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Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit anti-Human, Mouse Collagen IV alpha2 Polyclonal Antibody | anti-COL4A2 antibody

Collagen IV alpha2 Antibody

Gene Names
COL4A2; ICH; POREN2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
Collagen IV alpha2; Polyclonal Antibody; Collagen IV alpha2 Antibody; Collagen alpha-2(IV) chain; Canstatin; COL4A2; anti-COL4A2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Collagen IV alpha2 Antibody detects endogenous levels of total Collagen IV alpha2 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1,712
Applicable Applications for anti-COL4A2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen IV alpha2.
Immunogen Range
151-200
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from Jurkat cells, using Collagen IV alpha2 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from Jurkat cells, using Collagen IV alpha2 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
167 kDa
NCBI Official Full Name
collagen alpha-2(IV) chain preproprotein
NCBI Official Synonym Full Names
collagen type IV alpha 2 chain
NCBI Official Symbol
COL4A2
NCBI Official Synonym Symbols
ICH; POREN2
NCBI Protein Information
collagen alpha-2(IV) chain
UniProt Protein Name
Collagen alpha-2(IV) chain
Protein Family
UniProt Gene Name
COL4A2

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Research Articles on COL4A2

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Product Notes

The COL4A2 col4a2 (Catalog #AAA9505696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen IV alpha2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen IV alpha2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000. Researchers should empirically determine the suitability of the COL4A2 col4a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen IV alpha2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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