Rabbit anti-Human, Mouse JIP3 Polyclonal Antibody | anti-JIP3 antibody

JIP3 Antibody

Cat. Num. AAA9505547

• Gene Names: MAPK8IP3; syd; JIP3; SYD2; JIP-3; JSAP1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: JIP3; Polyclonal Antibody; JIP3 Antibody; C-jun-amino-terminal kinase interacting protein 3; JIP-3; JNK MAP kinase scaffold protein 3; JNK-interacting protein 3; JNK/SAPK-associated protein 1; JSAP1; KIAA1066; MAPK8IP3; Mitogen- activated protein kinase 8-interacting protein 3; SYD2; Sunday; anti-JIP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: JIP3 Antibody detects endogenous levels of total JIP3 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 250

• Applicable Applications for anti-JIP3 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:20000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human JIP3.
• Immunogen Range: 621-670
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using JIP3 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HeLa cells, using JIP3 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 94557295
• NCBI GeneID: 23162
• NCBI Accession #: NP_001035529.1
• NCBI GenBank Nucleotide #: NM_001040439.1
• UniProt Accession #: Q9UPT6; Q96RY4; Q9H4I4; Q9H7P1; Q9NUG0; A2A2B3; A7E2B3
• Molecular Weight: 147 kDa
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 3
• NCBI Official Symbol: MAPK8IP3
• NCBI Official Synonym Symbols: syd; JIP3; SYD2; JIP-3; JSAP1
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 3
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 3
• Protein Family: Putative uncharacterized protein
• UniProt Gene Name: MAPK8IP3
• UniProt Synonym Gene Names: JIP3; KIAA1066; JIP-3; JNK-interacting protein 3

NCBI Description

The protein encoded by this gene shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins ().